龙血竭对大鼠皮瓣存活影响的实验研究

The experimental study of the effect of Dracaena cochinchinesis （ Lour. ） S.C. Chen on survival of random skin flaps in rats

目的研究龙血竭对大鼠随意皮瓣存活的影响。方法取SPF级健康sD雄性大鼠30只，背部制作大小7．0cm×1．5cm的随意皮瓣，原位缝合。随机分为模型组和龙血竭组。龙血竭组每天灌胃给予龙血竭0．5％羧甲基纤维素钠（CMC—Na）混悬液（8g/kg）；模型组给予同体积的CMC—Na溶液。给药后7d测定两组皮瓣成活率，进行HE染色组织学观察和微血管密度（MvD）计数。检测皮瓣组织中超氧化物歧化酶（superoxidedismutase，SOD）、丙二醛（malonyldialdehyde，MDA）、表皮细胞生长因子（epidermal growth factor，EGF）和血管内皮生长因子（vascular endothelial growth factor，VEGF）含量。结果龙血竭组皮瓣成活率为（67．9±6．81）％，高于模型组（49．65±9．39）％。组织学观察表明龙血竭组中性粒细胞浸润较模型组明显减轻，皮层完整度、血管、成纤维细胞增生均优于模型组。龙血竭组MVD为（25．62±6．31）个高于模型组（20．76±4．11）个。龙血竭组皮瓣组织中SOD和EGF含量明显增加，而MDA水平明显降低，与模型组比较差异均有统计学意义（P〈0．05）。两组VEGF水平比较差异无统计学意义（P〉0．05）。结论龙血竭能提高随意皮瓣的成活率，作用机制与清除自由基、降低脂质过氧化反应、促进血管新生及改善血液供应有关。

Objective To study the influence of Dracaena cochinchinesis （Lour. ） S.C. Chen on the survival of random skin flap in rats. Methods A 7.0 cm × 1.5 cm random pattern skin flap was lifted on the back of 30 male Sprague Dawley rats, and then orthotopically sutured. These rats were randomly divided into two groups：the model group and Dracaena cochinchinesis （Lour.） S.C. Chen group （DC group）. Rats in the DC group were given Dracaena cochinchinesis （ Lour. ） S.C. Chen suspended in 0.5% sodium carboxymethyl cellulose （8 g/kg） by intragastric administration. Rats in the model group were given sodium carboxymethyl cellulose solution of the same volume. The flap survival rate was measured 7 days after model creation. Flap tissues were sampled for histological sections and HE staining to observe microvessel density. The levels of superoxide dismutase （SOD）, malonyldialdehyde （MDA）, epidermal growth factor （EGF）, and vascular endothelial growth factor （VEGF） were detected. Results The survival rate of flaps in the DC group was （67.91±6.81）%, being significantly higher than that in the model group which was （49.65 ± 9.39）%. Histological observations showed that, compared to tissues in the model group, tissues in the DC group had significantly reduced neutrophils infiltrating, better dermal integrity, and more vascular and llbroblast regeneration. Tissues in the DC group also had higher content of SOD and EGF and lower MDA level compared to tissues in the model group. However, there was no difference between the VEGF levels in the two groups. Conclusion Dracaena cochinchinesis （I-our.） S.C. Chen can improve the survival rate of random skin flap. Its mechanism is related to free radical scavenging, lipid peroxidation reduction, and enhanced angiogenesis and improved blood supply.