草胡椒属植物对人黑色素瘤A375细胞增殖的影响

Effect of Peperomia Ruizet Pav on Cell Proliferation of Human Malignant Melanoma A375 Cell

目的考察三种草胡椒属植物豆瓣绿、石蝉草和草胡椒乙醇提取物及不同极性部位对人黑色素瘤(A375)细胞增殖抑制作用。方法常规传代培养A375细胞,实验处理前后,采用噻唑蓝(MTT)法测定细胞活性。结果豆瓣绿、石蝉草和草胡椒的乙醇提取物对A375细胞增殖均有抑制作用,且细胞存活率随样品质量浓度的加大而降低。其中乙酸乙酯极性部位抑制效果更加明显,水层在高浓度下有一定的抑制作用。结论该属三种植物石蝉草、豆瓣绿和草胡椒均有抗肿瘤作用。

Objective Compare the inhibition effect on human melanoma cells（ A375） treated with the ethanolic extracts and different polar parts obtained from three different Peperomia Ruizet Pav species. Methods Human melanoma A375 cells were grown in DMEM medium,MTT assay was used to examine the growth rates inhibition on human melanoma cells. Results The ethanolic extracts of three different Peperomia Ruizet Pav species has inhibitory effects on A375 cell proliferation,and the survival rate reduced with the increase of sample mass concentration. The effect of the ethyl acetate polar parts is more apparent,water layer does little effect. Conclusion The three species of Peperomia Ruizet Pav all have antitumor effect.