钩吻二氯甲烷部位化学成分HPLC指纹图谱研究

Study on the HPLC fingerprint of chemical constituents in dichloromethane extraction of Gelsemium elegans

目的建立钩吻二氯甲烷部位的化学成分指纹图谱。方法采用RP-HPLC方法,依利特色谱柱(250 mm×4.6mm,5μm);流动相A为乙腈,流动相B为水(含0.3%磷酸-0.5%三乙胺);梯度洗脱;流速1 ml·min-1;柱温40℃;进样体积20μl;检测波长254 nm。并对钩吻二氯甲烷部位进行指纹图谱定性分析。结果 10份不同产地钩吻二氯甲烷部位的指纹图谱经《中药色谱指纹图谱相似度评价系统(2004A版)》分析相似度较高,共检出9个相对位置稳定的共有峰,通过与标准品的保留时间及紫外光谱比较,可鉴定为钩吻碱甲和钩吻碱丙,保留时间分别为17.18 min和32.52 min。结论钩吻药材二氯甲烷部位指纹图谱的建立为药材质量控制提供了科学依据。

Objective To establish the fingerprint of chemical constituents of dichloromethane extraction of Gelsemium elegans.Methods The RP- HPLC analysis was performed on an Elite C18（ 250 mm × 4. 6 mm,5 μm） column with gradient elution of acetonitrile-（ 0. 3% phosphoric acid- 0. 5% triethylamine） at a flow rate of 1. 0 m L·min- 1. The detection wavelength was set at 254 nm and the column temperature was set at 40℃. Results The fingerprint spectrum of dichloromethane extraction in 10 different batches of Gelsemium elegans showed high similarity through the software named ＂ Evaluation of similarity of the fingerprint spectram of Chinese herbs＂. HPLC showed totally 9 specific peaks of the dichloromethane extraction in Gelsemium elegans. By comparison of the retention time and the on- line UV spectra of chemical standards,gelsemine and sempervirine peak were identified. The retention time was 17. 18 min and 32. 52 min respectively. Conclusion This method can be used for quality control of Gelsemium elegans.