爆米花中双乙酰含量检测方法的建立及应用

Establishment and application of detection method of diacetyl content in popcorn

目的建立及优化爆米花中双乙酰含量的测定方法,并对潍坊地区市售爆米花中双乙酰含量进行检测。方法从爆米花预处理方法、料液比、蒸馏提取时间、显色时间及最佳检测波长等方面,建立并优化爆米花中双乙酰含量的检测方法,运用所建立的方法对6种市售爆米花中双乙酰含量进行测定。结果通过优化得到最佳提取工艺为:爆米花样品粉碎目数40目,料液比1∶5,蒸馏提取时间30 min,显色时间20 min,最佳检测波长335 nm,利用该方法测定6种市售爆米花中双乙酰的含量分别为7.15、10.85、5.05、7.50、7.40、6.25 mg/kg。结论试验建立的双乙酰含量的测定方法简单易行,有良好的回归性和可再现性,方法检出限为0.068 7μg/L。试验测得的潍坊地区6种市售爆米花中双乙酰含量均在美国香料和香精制造者协会(FEMA)规定范围内。

Objective To establish and optimize a method for determining diacetyl content in popcorn,and determine the diacetyl content in popcorn sold in Weifang.Methods Popcorn pretreatment,diacetyl distillation extraction time,and absorption spectral scan condition were optimized to establish the method for the determination of diacetyl content inpopcorn.Diacetyl content of 6 kinds of popcorns was determined with the method.Results The optimum extraction conditions of diacetyl content in popcorn were 40 meshes material granularity,1∶5 materiel-liquid ratio,30 min extraction time,20 min reaction time,and 335 nm wavelength detection.The diacetyl content of 6 kinds of popcorns was 7.15,10.85,5.05,7.50,7.40 and 6.25 mg/kg respectively.Conclusion The method is simple and easy to operate,and it has good regression and reproducibility.The diacetyl content of 6 kinds of popcorns was within the prescribed scope of Flavour Extract Manufacturers＇ Association（FEMA）.