溴氰菊酯对马铃薯腐烂茎线虫的毒力及对其运动和摄食的影响

Toxicity of deltamethrin on Ditylenchus destructor and the effects on its locomotion and feeding behaviors

采用药液浸渍法、沙柱法以及与荧光染料Cy3共孵育的方法,以丙溴磷、克百威和阿维菌素为对照药剂,测定了溴氰菊酯对马铃薯腐烂茎线虫Ditylenchus destructor的毒力以及对其运动扩散和摄食的影响。结果表明:溴氰菊酯对马铃薯腐烂茎线虫具有一定的杀灭活性,其LC_(50)值为459.8 mg/L,活性低于丙溴磷(159.9 mg/L)、克百威(331.9 mg/L)和阿维菌素(257.3 mg/L);溴氰菊酯对马铃薯腐烂茎线虫运动扩散的抑制作用IC_(50)值为3.1 mg/L,其活性低于阿维菌素(0.8 mg/L),但高于丙溴磷(8.3 mg/L)和克百威(16.1 mg/L)。当丙溴磷和克百威质量浓度分别低至0.6和40 mg/L时,可刺激90%以上的线虫摄食;当丙溴磷和克百威质量浓度最低分别为360和300 mg/L时,可抑制全部线虫的摄食;溴氰菊酯与阿维菌素则对线虫的摄食无刺激作用;用10 mg/L的丙溴磷处理线虫2 h后再分别用阿维菌素和溴氰菊酯处理,发现阿维菌素和溴氰菊酯质量浓度最低分别为20和200 mg/L时可抑制全部马铃薯腐烂茎线虫的摄食。研究表明,溴氰菊酯对马铃薯腐烂茎线虫具有较高的活性,其在线虫防治领域的开发应用潜力较好。

Effects of deltamethrin on the mortality, locomotion and feeding behaviors of Ditylenchus destructor were investigated by dipping method, sand column method and incubating fluorescent Cy3 method, using profenofos, carbofuran and abamectin as control. The results showed that deltamethrin had moderate nematicidal activity against D. destructor, with LC_（50） value of 459.8 mg/L, while the LC_（50） values of profenofos, carbofuran and abamectin to D. destructor were 159.9, 331.9 and 257.3 mg/L,respectively. Deltamethrin also showed better inhibitive activity on the locomotion of D. destructor,with IC_（50） value of 3.1 mg/L, which was higher than that of profenofos（8.3 mg/L） and carbofuran（16.1mg/L）, but lower than that of abamectin（0.8 mg/L）. In the case of feeding, the minimal concentration of profenofos that stimulated more than 90% nematode intake of Cy3 was 0.6 mg/L, and that of carbofuran was 40 mg/L. The minimal concentration of profenofos that inhibited all of the nematode intake of Cy3 was 360 mg/L, and that of carbofuran was 300 mg/L. Deltamethrin and abamectin could not stimulate the feeding of D. destructor. After exposure to 10 mg/L profenofos for 2 h, abamectin and deltamethrin could inhibit all of nematodes intake of Cy3 at 20 and 200 mg/L, respectively. The results indicated that deltamethrin have the potential in the control of D. destructor.