黄芪与红芪SSR引物的筛选及鉴定指纹代码的构建

Astragali Radix and Hedysari Radix molecular identification of SSR primers screening and fingerprints code

收集豆科SSR引物,通过分子标记技术,筛选验证可用于黄芪与红芪鉴定的核心引物。结果表明,101对SSR引物中筛选出6对有效鉴定引物,其PCR产物在相对分子质量100-500 bp,可形成的7-12条数目不等的电泳条带,其中多态性位点55个,多态性比率为100%,平均多态信息含量为0.371,多态位点建立的聚类分析（UPMGA）结果显示,获得的核心引物可在相似度为0.4处100%区分62份黄芪与红芪的混合样品,标记参试引物及对应的特征泳带,生成指纹图谱代码,为黄芪与红芪的鉴别提供依据。

Leguminous related SSR primers were collected,core primers used for Astragali Radix and Hedysari Radix identification were screened and validated by using molecular marker techniques. 6 core primers were selected from 101 pairs of primers,the molecular weight of PCR products was 100-500 bp,which formed 7-12 electrophoresis bands with 55 amplified loci. The percentage of polymorphic loci was 100%,and the average polymorphism information content was 0. 371. According to the results of cluster analysis,obtained core primer could completely distinguish 62 mixture samples of Astragali Radix and Hedysari Radix in similarity coefficient of0. 46. Core primers and the corresponding characteristics from gel electrophoresis were tagged. The results provide identification basis for Astragali Radix and Hedysari Radix.