昆明柏化学成分研究

Chemical Constituents of Sabina gaussenii

目的：研究昆明柏Sabina gaussenii枝叶中的化学成分。方法：应用硅胶,羟丙基葡聚糖凝胶（Sephadex LH-20）,RP-18,MCI-gel柱色谱和半制备HPLC等手段进行分离纯化;根据理化性质和波谱学方法鉴定化合物的结构。结果：从昆明柏枝叶乙醇提取物的乙酸乙酯萃取部位中分离得到7个化合物,分别鉴定为（7S,8R）-dihydro-3-hydroxy-8-hydroxy-methyl-7-（4-hydroxy-3-methoxyphenyl）-1-benzofuranpropanol（1）,（8R,8＇S,7S）-4＇-（3″-methoxyrhamnopyranosyl）oxy-8＇-hydroxy-3,3＇,4-trimethoxy-8-hydroxymethyl-lign-7-9＇-lactone（2）,7S,8R-erythro-4,7,9,9＇-tetrahydroxy-3,3＇-dimethoxy-8-O-4＇-neolignan（3）,7S,8Sthreo-4,7,9,9＇-tetrahydroxy-3,3＇-dimethoxy-8-O-4＇-neolignan（4）,leucoseceptoside A（5）,焦地黄苯乙醇苷D（6）,blumenol A（7）。结论：化合物1-7均为首次从该植物中分离得到。

Objective： To study the chemical constituents from the branches and leaves of Sabina gaussenii. Method： Silica gel, Sephadex LH-20, RP-18, MCI-gel column chromatography, semi-preparative HPLC and other means were used to isolate an purify the constituents, the structures of the compounds were identified by physicochemical properties and spectral data. Result： Seven compounds were obtained from the ethyl acetate fraction of ethanol extract in the branches and leaves of S. gaussenii and identified as（ 7S,8R）-dihydro-3-hydroxy-8-hydroxy-methyl-7-（ 4-hydroxy-3-methoxyphenyl）-1-benzofuranpropanol（ 1）,（ 8R,8＇ S,7S）-4＇-（ 3″-methoxyrhamnopyranosyl） oxy-8＇-hydroxy-3,3＇,4-trimethoxy-8-hydroxymethyl-lign-7-9＇-lactone（ 2）,7S,8R-erythro-4,7, 9, 9＇-tetrahydroxy-3, 3＇-dimethoxy-8-O-4＇-neolignan（ 3）, 7S, 8S-threo-4, 7, 9, 9＇-tetrahydroxy-3,3＇-dimethoxy-8-O-4＇-neolignan（ 4）,leucoseceptoside A（ 5）,coke to yellow benzene glycosides D（ 6）,and blumenol A（ 7）. Conclusion： Compounds 1-7 were all obtained from S. gaussenii for the first time.