RP-HPLC同时测定硬尖神香草中4种成分含量

Simultaneous Determination of Four Constituents in Hyssopus cuspidatus by RP-HPLC

目的:建立维吾尔族药材硬尖神香草中槲皮素、咖啡酸、木犀草素、金合欢素4种成分的含量测定方法。方法:使用Agilent C18色谱柱(4.6 mm×250 mm,5μm),流动相甲醇-0.2%乙酸水溶液(30∶70),检测波长340 nm,流速1.0 m L·min^(-1),柱温30℃,进样量10μL。结果:槲皮素、咖啡酸、木犀草素、金合欢素分别在0.120 2~1.081 8 mg(r=0.998 6),0.031~0.279 mg(r=0.999 9),0.052~0.468 mg(r=0.998 9),0.098 1~0.882 9 mg(r=0.998 8)呈良好的线性关系,回收率分别为98.62%(RSD 1.4%),97.38%(RSD 1.7%),99.41%(RSD 1.5%),99.81%(RSD 1.0%),结论:采用RP-HPLC同时测定硬尖神香草中4种成分含量方法,该方法可行且简单有效,可作为硬尖神香草含量测定的理论指标。

Objective： To establish a method for determining the contents of quercetin,caffeic acid,luteolin,and acacetin in Uygur Medicine Hyssopus cuspidatus. Method： Agilent C18 chromatographic column（ 4. 6 mm × 250 mm,5 μm） was used with methanol-0. 2% acetic acid solution as the mobile phase. The detection wavelength was set at 340 nm and the flow rate of 1. 0 m L·min^-1. The column temperature was 30 ℃ and the injection volume was 10 μL. Result： Quercetin, caffeic acid, luteolin, and acacetin showed good linear relationship in 0. 120 2-1. 081 8 mg（ r = 0. 998 6）,0. 031-0. 279 mg（ r = 0. 999 9）,0. 052-0. 468 mg（ r = 0. 998 9）,and 0. 098 1-0. 882 9 mg（ r = 0. 998 8） respectively,and their recovery rate was 98. 62%（ RSD1. 4%）,97. 38%（ RSD 1. 7%）, 99. 41%（ RSD 1. 5%）, and 99. 81%（ RSD 1. 0%） respectively.Conclusion： This RP-HPLC method is feasible and simple for simultaneous determination of 4 components in H. cuspidatus,which can provide theoretical indicators for the content determination of H. cuspidatus.