臭氧处理对糯米中氨基酸组成的影响

Effect of ozone treatment on the amino acid composition of glutinous rice

以臭氧为氧化剂,直接干法处理糯米后提取蛋白质,检测糯米蛋白质在臭氧的氧化作用下巯基、二硫键及氨基酸含量的变化,研究臭氧氧化处理对糯米制备蛋白质中氨基酸组成的影响。实验结果表明:糯米蛋白质在臭氧的持续氧化处理下巯基含量逐渐减少,二硫键含量呈增加趋势;臭氧处理使氨基酸总含量减少,其中含硫类氨基酸较对照组下降幅度最大,芳香类氨基酸含量较对照组下降程度次之,疏水性氨基酸下降程度较小,其他类型的氨基酸含量受臭氧处理的影响微弱。

In order to study the effect of ozone treatment on the amino acid composition of glutinous rice, ozone was used as an oxidant to treats the protein which extracted from the dry processed glutinous rice. And the sulfhydryl content, disulfide bond content and amino acid content of the extracted protein were measured. The experiment data indicated that the sulfhydryl content of glutinous rice was reducing as the ozone treating, while the disulfide bond content was increasing. Simultaneously, the total amino acid content of the extracted protein reduced after ozone treatment. In which, the sulfur amino acids content reduced most, the aromatic amino acids reduced second and the hydrophobic amino acids reduced little The other types of amino acids contents were affected little by ozone treatment.