牙龈卟啉单胞菌对牙周膜成纤维细胞活性、炎性因子与骨代谢基因表达的影响研究

Effects of viable Porphyromonas gingivalis on the viability and gene expression of inflammatory cytokines and bone metabolism of periodontal ligament fibroblasts

目的探讨牙龈卟啉单胞菌（P.gingivalis）活菌感染牙周膜成纤维细胞（PDLF）后对细胞活性、炎性因子和骨代谢相关基因表达的影响。方法 P.gingivalis活菌分别以10^9、10^8、10^7、10^6、10^5CFU/ml浓度感染PDLF 6 h,检测细胞活性和白细胞介素-6（IL-6）、IL-8、IL-1β、肿瘤坏死因子-α（TNF-α）、RANKL、骨保护素（OPG）的基因表达。结果 P.gingivalis攻击PDLF 6 h后,细胞活性差异无统计学意义。与对照组比较,P.gingivalis浓度分别达到108CFU/ml和107CFU/ml时,IL-6和IL-8基因表达上升,差异有统计学意义（P〈0.01）,P.gingivalis浓度达到109CFU/ml时,IL-1β、TNF-α基因表达上升,差异有统计学意义（P〈0.01）。各实验组OPG基因表达均下降,差异有统计学意义（P〈0.01）,RANKL基因表达差异无统计学意义。结论 P.gingivalis活菌感染PDLF后,可产生一系列的细胞因子,参与牙周组织的破坏与改建。

Objective To study the cell viability and gene expression of inflammatory cytokines and bone metabolism of periodontal ligament fibroblasts（ PDLF） upon challenge by viable Porphyromonas gingivalis（ P. gingivalis）.Methods Human PDLF was challenged in vitro by viable P. gingivalis for 6 hours and then the cell viability and gene expression of inflammatory cytokines such as IL-6,IL-8,IL-1β,TNF-α,RANKL,OPG were analyzed. Results No obvious change in cell viability was observed before and after challenged by viable P. gingivalis. The expressions of both IL-6 and IL-8 were strongly induced when the concentration of viable P. gingivalis was 108 CFU /ml and 107 CFU / ml. The expression of IL-1β and TNF-α also increased significantly when the concentration of viable P. gingivalis was 10^9 CFU / ml. The expression of OPG was suppressed significantly by P. gingivalisin in PDLF while the gene expression of RANKL remained unchanged. Conclusion When challenged by P. gingivalis,PDLF participates in the regeneration and remodeling of periodontal tissue by producing cytokines.