四川都江堰川芎根茎真菌的分离与鉴定

Isolation and Identification of Ligusticum Chuanxiong Rhizome Fungi of Dujiangyan in Sichuan

探究川芎内生真菌、附生真菌的种类,为川芎病害防治提供理论基础。本试验采用组织分离的方法,对采得的30份都江堰川芎根茎样品进行分离,鉴定其内生真菌和附生真菌。结果表明:分离得到10种真菌,其中内生真菌4种,附生真菌9种。内生真菌以镰孢属和木霉属为主,附生真菌中木霉、镰刀菌和毛霉为主,内生、附生真菌以木霉和镰刀菌为主要种群,附生真菌数量及种群组成均比内生真菌丰富,并在附生真菌中发现丛梗孢属,在以往研究川芎附生真菌的报道中均未见。

In order to provide a theoretical basis for disease control on Chuanxiong,the species of endophyte and epiphytic fungi on Chuanxiong were explored in this paper. Organizational separation method was used to identify the species of endophyte and epiphytic fungi on 30 Chuanxiong rhizome samples in Dujiangyan,Sichuan. Results showed that 10 kinds of fungi were isolated from the samples,including four kinds of endophyte,nine kinds of epiphytic fungi. Fusarium and Trichoderma were mainly endophyte while Trichoderma,Fusarium and Mucor were mainly endogenous epiphytic fungi,and Trichoderma and Fusarium were the main populations of epiphytic endophytic fungi and fungal species composition were much rich than that of endophyte,and Monilia was found in epiphytic fungi,which was never reported in relevant epiphytic fungi study on Chuangxiong.