摘要
目的:检测小窝蛋白-1(Cav-1)和E-钙黏蛋白(E-cad)在食管癌TE1细胞系中的表达水平,探讨Cav-1在食管癌转移中可能的作用及机制。方法:化学合成针对Cav-1基因3个不同靶点的siRNA(Cav-1 siRNAl,Cav-1 siRNA2和对照siRNA),分别转染食管癌TE1细胞系,以未转染细胞为空白对照,转染对照siRNA细胞为阴性对照。采用Western blot法检测RNA干扰后TE1细胞中Cav-1和Ecad的表达情况;Transwell方法检测各组细胞的迁移和侵袭能力。结果:Western blot检测结果提示,与空白对照组比较,Cav-1siRNA使食管癌TE1细胞系中Cav-1表达水平降低(P<0.01),E-cad表达水平升高(P<0.01);Transwe迁移及侵袭试验结果提示转染Cav-1siRNA后细胞的迁移、侵袭能力较空白对照组显著减弱(P<0.05)。结论:Cav-1可能通过调节E-cad的表达影响食管癌的转移。
OBJECTIVE:To detect the expression of Caveolin-l(Cav-l) and E-cadherin(E-cad) in esophageal carcinoma TE1 cell line and to explore the involvement of Cav-1 in metastasis of esophageal carcinoma.METHODS:Small interfering RNAs Cav-1 siRNAl and Cav-1 siRNA2 which targeted Cav-1 and negative control siRNA were individually transfected into esophageal carcinoma TE1 cell line.Non-transfected cells were used as blank control.Expressions of Cav-1 and E-cad were detected in cell extracts using Western blot.Migration and invasion ability of cells were evaluated using the Transwell assay.RESULTS:The Western blot analysis showed that Cav-1 siRNAs effectively suppressed the expression of Cav-1 and up-regulated the expression of E-cad in TE1 cells.The transwell assay showed that the migration and invasion ability of Cav-1 siRNA treated group were significantly weakened compared with the blank control group(P〈0.05).CONCLUSION:Cav-1 may affect metastasis of esophageal carcinoma by up-regulating the expression of E-cad.
出处
《癌变.畸变.突变》
CAS
CSCD
2016年第3期227-230,共4页
Carcinogenesis,Teratogenesis & Mutagenesis