布地奈德对哮喘大鼠肺组织14-3-3蛋白及14-3-3β mRNA表达的影响

Effect of Budesonide on 14-3-3 Protein and 14-3-3β mRNA Expression in Asthmatic Rat Lung Tissue

目的:观察14-3-3蛋白及14-3-3βmRNA在哮喘大鼠肺组织中的表达,探讨布地奈德的抗炎作用机制。方法:利用鸡卵白蛋白(OVA)复制哮喘模型,分别采用免疫组化法和实时荧光定量PCR法检测肺组织14-3-3蛋白及14-3-3βmRNA的表达水平。结果:哮喘组肺组织14-3-3蛋白OD值为0.353±0.023,14-3-3βmRNA OD值为1.78±0.36,显著高于对照组0.211±0.028、1(P<0.01)。布地奈德组14-3-3蛋白OD值为0.310±0.024,显著低于哮喘组(P<0.01);14-3-3βmRNA(1.39±0.51)的表达水平与哮喘组(1.78±0.36)比较差异无统计学意义(P>0.05)。结论:哮喘急性期14-3-3蛋白及14-3-3βmRNA呈现过度表达,可能发挥促炎的作用,但这种表达可以被布地奈德抑制。

Objective： To investigate the possible anti-inflammation roles of budesonide,observe the expressions of 14-3-3 protein and14-3-3β mRNA. Methods： Rats asthma models were challenged by ovalbumin,and the expressions of 14-3-3 protein and 14-3-3βmRNA were determined respectively by immunohistochemisty methods and real-time PCR methods at lung tissue. Results： The expressions of 14-3-3 protein and 14-3-3β mRNA in asthma group（ 0. 353± 0. 023 and 1. 78± 0. 36 optical density,respectively） were significantly higher than those in control group（ 0. 211 ± 0. 028 and 1 optical density,respectively）（ P 0. 01）. Noteworthy,the expression of 14-3-3 protein was significantly decreased in budesonide group（ 0. 310±0. 024 optical density） than that in asthma group（ P 0. 01）. Nevertheless,the expressions of 14-3-3β mRNA were not significantly different in budesonide group（ 1. 39±0. 51） and asthma group（ 1. 78±0. 36）（ P 0. 05）. Conclusion： The expressions of 14-3-3 protein and 14-3-3β mRNA were overexpressed in asthmatic lung tissue,it may act as a pro-inflammation mediator,but this can be suppressed by budesonide.