药用植物三楞草的寡糖成分研究

Study on Oligosaccharide Constituents of Medicinal Plants Campylotropis Trigonoclada

目的:对药用植物三楞草的寡糖化学成分进行研究,以期寻找活性成分。方法:运用硅胶、ODS以及凝胶柱分离三楞草水部位,通过NMR技术鉴定其结构。结果:从三楞草水部位分离得到4个寡糖成分,分别是[O-α-D-glucopyranosyl-(1→4)]2-O-β-D-fructofuranosyl-(2→1)-α-D-glucopyranoside(1)、O-α-D-glucopyranosyl-(1→2)-O-[β-D-fructofuranosyl-(2→1)]2-α-D-glucopyranoside(2)、[O-α-D-glucopyranosyl-(1→2)]2-O-[β-D-fructofuranosyl-(2→1)]2-α-D-glucopyranoside(3)和O-α-D-glucopyranosyl-(1→2)-O-[β-D-fructofuranosyl-(2→1)]3-α-D-glucopyranoside(4)。结论:这4种寡糖成分首次从该药用植物中分离得到。

〔Abstract〕Objective： To explore the oligosaccharide constituents of Campylotropis Trigonoclada for finding bioactive natural products. Methods：The extract water part of C. trigonoclada was chromatographied by silica gel, ODS column and LH-20 gel column chromatography methods, and the isolates were identified based on spectroscopic analysis （1H NMR and 13C NMR）. Results： Four oligosaccharide compounds were isolated from C. trigonoclada and characterized as[O-α-D-glucopyranosyl-（1→4）]2-O-β-D-fructofuranosyl-（2→1）-α-D-glucopyranoside（1）, O-α-D-glucopyranosyl-（1→2）-O-[β-D-fructofuranosyl-（2→1）]2-α-D-glucopyranoside（2）,[O-α-D-glucopyranosyl-（1→2）]2-O-[β-D-fructofuranosyl-（2→1）]2-α-D-glucopyranoside（3）and O-α-D-glucopyranosyl-（1→2）-O-[β-D-fructofuranosyl-（2→1）]3-α-D-glucopyranoside （4）. Conclusion： Four oligosaccharide compounds were isolated from this genus for the first time.