云南部分兰科植物多糖的提取及含量测定

Extraction of Polysaccharide and Content Determination of Yunnan Orchidaceae Plants

目的：建立云南部分兰科植物多糖含量测定的方法，并对其提取工艺进行研究。方法：采用传统水提取方法提取多糖，以葡萄糖为标准品，用苯酚-硫酸法测定多糖的含量，并以其为考察指标，采用提取条件的优化筛选最佳提取工艺。结果：葡萄糖在0.000~0.020 mg/mL范围内线性关系良好，线性回归方程为A=55.55c（mg/mL）＋0.001（ r=0.9998），平均加标回收率为99.7%，RSD=3.13%（n=6）；最佳显色条件：苯酚用量1.0 mL、浓硫酸用量7.0 mL、显色温度80℃、显色时间30 min；提取兰科植物多糖的最佳工艺条件为：提取温度70℃，料液比1：40，提取时间2 h。结论：该方法简便、准确，优选出的提取工艺重复性好。

Objective： To establish the method for determining the contents of polysaccharides and to explore the extraction technique for polysaccharides from Yunnan orchidaceae plants. Methods：The polysaccharide was extracted with the traditional water extraction, using glucose as the standard, and the contents of polysaccharides was measured by the phenol-sulfuric acid method. With the content of polysaccharides as an index, the univariate test were performed to find the optimal extraction technique. Results：A good linear relationship with a regression equation of A=55.55c（mg/mL）＋0.001（r=0.999 8）was achieved when the concentration of glucose ranged between 0.000 and 0.020 mg/mL. The average recovery rate was 99.7%, and the relative standard deviation was 3.13%（n=6）. The best conditions of color are as follows, the dosage of phenol and sulphuric acid was 1.0 mL and 7.0 mL respectively, temperature of color was at 80℃, and developing time was 30 minutes. The optimal extraction of polysaccharide was performed by 2 hours soaking using 1：40 volumes of solvent at 70 ℃. Conclusion： The established method is simple and accurate and the optimal extraction technique shows good reproducibility.