阿霉素肾病肾阳虚证模型的评价

Evaluation of adriamycin-induced nephropathy model with syndrome of deficiency of kidney yang

目的:探讨阿霉素肾病肾阳虚证病证结合模型的技术方法,为肾脏病的病证结合研究提供技术支撑。方法:40只SD大鼠,按体质量随机分为正常组、阿霉素肾病模型组、阿霉素肾病肾阳虚证模型组、肾阳虚证模型组,分别采用阿霉素、氢化可的松建立阿霉素肾病模型、阿霉素肾病肾阳虚证模型、肾阳虚证模型,并于造模第2、3周末分别测各组体质量、肛温、24h尿蛋白定量、尿-17羟类固醇,第3周末测各组血肌酐、总胆固醇、白蛋白。结果:第2、3周末,各模型组体质量低于正常组(P<0.05,P<0.01),阿霉素肾病模型组、阿霉素肾病肾阳虚证模型组24h尿蛋白定量高于正常组及肾阳虚模型组(P<0.01);第3周末,阿霉素肾病模型组、阿霉素肾病肾阳虚证模型组血白蛋白低于正常组及肾阳虚模型组(P<0.01)、总胆固醇高于正常组及肾阳虚模型组(P<0.01),尿17-羟皮质类固醇含量高于与正常组及肾阳虚模型组(P<0.01)。结论:阿霉素肾病模型的建立是可行的,需进一步评价肾阳虚证模型的建立,为评价阿霉素肾病肾阳虚证结合模型创造条件。

Objective： To develop adriamycin-induced nephropathy model with syndrome of deficiency of kidney yang, a combination of disease and syndrome animal model, in order to provide technical support for the combination of disease and syndrome in renal disease. Methods： Forty SD rats were randomly divided into the normal group, adriamycin-induced nephropathy model group, adriamycin-induced nephropathy with syndrome of deficiency of kidney yang model group, and the syndrome of deficiency of kidney yang model group. The nephropathy models were established by adriamycin and hydrocortisone, respectively. The body weight, anal temperature, 24-hour proteinuria, and urine 17-hydroxycorticosteroid were observed at the 2th and 3th weekends after modeling. And, the serum creatinine, cholesterol, and albumin were detected at the 3th weekend after modeling. Results： At the 2th and 3th weekends, compared with normal group, the body weight in model groups were significantly decreased（P〈0.05, P〈0.01）. And, compared with the normal group, 24-hour proteinuria both in the adriamycin-induced nephropathy model group and adriamycin-induced nephropathy with syndrome of deficiency of kidney yang model group were significantly increased（P〈0.01）. At the 3th weekend, compared with the normal group, serum albumin both in the adriamycin-induced nephropathy model group and adriamycin-induced nephropathy with syndrome of deficiency of kidney yang model group were significantly decreased（P〈0.01）, while the serum cholesterol and urine 17-hydroxycorticosteroid increased（P〈0.01）. Conclusion： Adriamycininduced nephropathy model is feasible, however, the syndrome of deficiency of kidney yang model needs further evaluation, so as to provide support for the evaluation of adriamycin-induced nephropathy with syndrome of deficiency of kidney yang.