PSORI-CM01方抗银屑病的体内外药效考察

Pharmacodynamic study of PSORI-CM01 Formula on anti-psoriasis in vivo and in vitro

目的:观察PSORI-CM01方对银屑病模型动物的药效和对角质形成细胞HaCaT增殖抑制作用,考察其抗银屑病疗效。方法:将小鼠随机均分为正常组、模型组、阳性对照组(甲氨蝶呤片)、PSORI-CM01方低、中、高剂量组,口服给药,连续7d或14d,空白组和模型组给予等量纯净水,考察PSORI-CM01方对雌激素期小鼠阴道上皮细胞的有丝分裂指数及小鼠尾部表皮鳞片中颗粒层鳞片数的影响;用MTT法检测不同浓度和时间PSORI-CM01方对HaCaT细胞增殖的抑制率。结果:PSORI-CM01方可显著抑制雌激素期小鼠阴道上皮细胞的有丝分裂(P<0.01),其中、高剂量组略优于阳性对照组;显著促进小鼠尾部鳞片表皮颗粒层的形成(P<0.05,P<0.01),其低、中剂量组与阳性对照组相当;细胞实验显示PSORI-CM01方对共同培养24、48、72h的HaCaT细胞均有一定抑制作用,24、48、72h的IC50分别为1.23、1.16、0.50g/L。结论:PSORI-CM01方可从多个方面改善银屑病表现,具有良好开发前景。

Objective： To observe the drug effect of PSORI-CM01 Formula on psoriasis animal model and the inhibition of the proliferation of HaCaT keratinocyte cell line. Methods： Mice were randomly divided into blank group, model group, positive control group（methotrexate tablets）, low dose group, medium dose group and high dose group of PSORI-CM01 Formula. The treatment groups were orally administered with corresponding drugs, continuously one or two weeks, while the blank group and model group administered with the same volumes of pure water. The effect of PSORI-CM01 Formula on vaginal epithelium＇s mitosis index of mouse during estrogen period as well as the scale amount in the granular layer of mouse tail epidermis were used to evaluate the anti-psoriasis effect. Meanwhile, what effect of PSORI-CM01 Formula at different concentration and time would have on the proliferation of HaCaT keratinocyte cell line was observed by MTT. Results： PSORI-CM01 Formula significantly inhibited vaginal epithelium＇s mitosis of mouse during estrogen period（P〈0.01）, especially for the middle and high dose groups that were slightly better than the positive control group. It enhanced significantly the formation of scale amount in the granular layer of mouse tail epidermis（P〈0.05, P〈0.01）, especially for the low and middle dose groups that were equivalent to the positive control group. MTT assay revealed that PSORI-CM01 inhibited the proliferation of HaCaT keratinocyte cells in a dose-dependent manner（2.5, 1.25, 0.31g/L） during co-cultured 24 h, 48 h and 72 h, with the IC50 at 1.23, 1.16, 0.50g/L, respectively. Conclusion： PSORI-CM01 Formula could improve the development of psoriasis in many ways, so PSORI-CM01 Formula would be a promising drug in the treatment of psoriasis.