Iniferter自由基聚合法制备谷胱甘肽分子印迹聚合物及其吸附性能研究

Preparation and Adsorption Properties of Glutathione Molecularly Imprinted Polymers Synthesized via Iniferter Radical Polymerization

以谷胱甘肽(GSH)为模板分子,4-乙烯基吡啶(4-VP)为功能单体,乙二醇二甲基丙烯酸酯为交联剂,采用Iniferter自由基聚合法成功制备了谷胱甘肽分子印迹聚合物(L-MIPs)。利用计算机分子模拟和紫外光谱分析对模板分子和功能单体形成的复合物进行了系统研究,结果表明GSH与4-VP之间的相互作用力为氢键作用,其最佳配比为1:6。研究了溶剂种类对L-MIPs的结合性能和印迹因子的影响,发现二甲亚砜为制备L-MIPs的最佳溶剂。对比研究了L-MIPs与传统自由基聚合法制备的谷胱甘肽分子印迹聚合物(H-MIPs)的静态吸附性能、动态吸附性能及选择识别性能。结果表明,L-MIPs对GSH分子具有高度亲和性、快速结合性能和良好的选择识别性能。

Molecularly imprinted polymers（L-MIPs） were prepared via iniferter radical polymerization using glutathione（GSH） as a template molecule, 4-vinylpridine（4-VP） as a functional monomer and ethylene glycol dimethacrylate as a cross-linker. The functional monomer-template molecular（GSH-4-VP） complexes obtained were characterized by ultraviolet absorption spectroscopy and computer simulation. The results show that the GSH-4-VP complexes are formed via hydrogenbonding interactions, and the optimum molar ratio of GSH and 4-VP is 1：6. In addition, the effects of solvent type on L-MIPs binding properties and imprinting factors were studied. The results indicate that the optimal reaction solvent is dimethyl sulfoxide. Batch static binding tests and dynamic binding tests were used to estimate GSH binding properties and L-MIPs selective recognition. The results show that L-MIPs have high affinity, rapid binding rate and excellent selectivity when comparing with GSH imprinted polymers（H-MIPs） prepared using traditional radical polymerization methods.