黄腐酸处理对苹果树腐烂病菌的抑制作用及对苹果树防御酶活性的影响

Inhibition of fulvic acid to Valsa mali and its effect on the activity of defense enzyme in apple tree

黄腐酸作为一种广谱性植物生长调节剂,具有稳定可靠的抗逆增产效果。本试验以黄腐酸为生长调节剂,研究其对苹果树腐烂病菌(Valsa mali)的抑制作用及对苹果树皮抗腐烂病的影响。试验结果表明,黄腐酸能抑制苹果树腐烂病菌菌丝的生长,浓度越大抑制效果越明显。经黄腐酸处理后,苹果树腐烂病组织中SOD、POD、PAL和CAT均呈现先升高后降低的趋势。其中黄腐酸处理后SOD活性于14d达到峰值,而其他酶活性均在21d达到最大值。说明黄腐酸能显著提高苹果树体内相关防御酶系的活性,很大程度上提高了植物的免疫力,即增强了苹果树的抗病性。

As one of the broad-spectrum plant growth regulators,fulvic acid can improve crop resistance and increase the yield stably and reliably.The inhibition of fulvic acid against Valsa mali and its effect on the activity of defense enzymes,including SOD,POD,PPO,PAL and CAT,were determined.The results showed that fulvic acid could inhibit the growth of V.mali hyphae,and the inhibition effect increased with the increase of concentration.The activities of SOD,POD,PPO,PAL and CAT increased at first and then decreased smoothly after treatment by fulvic acid.The SOD activity reached the peak14 days after treatment,and the activity of other defense enzymes reached the maximum21 days after treatment.These results revealed that fulvic acid could significantly increase the activity of the tested defense enzymes in apple trees,and improve the immunity and disease resistance of the apple tree.