棉花枯萎病新杀菌剂毒力测定中助溶剂的筛选

The Cosolvent Screening of New Fungicides in Cotton Fusarium Wilt Toxicity Test

为了筛选对棉花枯萎病新杀菌剂毒力测定没有干扰的安全助溶溶剂。采用生长速率法测定了3种有机溶剂对棉花枯萎病菌的毒力和安全性。结果表明：丙酮、DMF和DMSO对棉花枯萎病菌的EC50值分别是161395.4、2704.8、1848.5 mg/L,以丙酮的毒力最低;其相对安全的质量浓度区间分别为0~161395.4、0~2704.8、0~1848.5 mg/L,抑菌率分别为0~9.2%、0~22.2%、0~6.8%,以丙酮最为安全。因而首选安全助溶有机溶剂为丙酮,其安全质量浓度区间为0~50000 mg/L。

In order to filter out the security cosolvent which has no interference in cotton Fusarium Wilt toxicity test of new fungicides,we adopt the growth rate method testing the toxicity and safety of three kinds of organic solvents for cotton Fusarium Wilt. The rusults show that the EC50 value of acetone,N,N- dimethyl formamide（ DMF） and dimethyl sulfoxide（ DMSO） of cotton Fusarium Wilt are 161395. 4,2704. 8,1848. 5 mg / L,respectively,the toxicity of acetone is the lowest; their mass concentration interval of relative safety are 0 ~ 161395. 4,0 ~ 2704. 8,0 ~ 1848. 5 mg /L,the bacteriostatic ratio are 0 ~ 9. 2%,0 ~ 22. 2%,0 ~ 6. 8%,respectively,the acetone is the most safe. Thus,the first choice of security organic cosolvent is acetone,its safe mass concentration interval is 0 ~ 50000 mg / L.