淫羊藿、黄芪、葛根有效组分复方对阿尔茨海默病转基因模型小鼠大脑皮质DMT1表达的影响

Effects of effective fraction of Epimedium,Astragalus,Radix Puerariae on the expression of DMT1 in the cerebral cortex of transgenic mice model of Alzheimer′s disease

目的探讨淫羊藿、黄芪、葛根有效组分复方对阿尔茨海默症(AD)APPswe/PS1ΔE9双转基因小鼠模型大脑皮质二价金属离子转运蛋白1(DMT1)表达的影响。方法将APPswe/PS1ΔE9双转基因小鼠60只,分为模型组、淫羊藿组、黄芪组、葛根组、复方组、去铁胺(DFO)组,C57BL/6J小鼠作为健康对照组。用药结束后,取各组小鼠脑组织,分别采用免疫组织化学、实时荧光定量PCR(RT-PCR)和蛋白免疫印迹法(Western blot)检测各组小鼠大脑皮质DMT1的表达情况。结果免疫组织化学结果显示:阴性对照未见DMT1阳性细胞。与健康对照组相比,模型组DMT1的表达增高;与模型组相比,复方组和DFO组DMT1的表达降低(P<0.05);DFO组与复方组DMT1的表达无明显差异。RT-PCR结果、Western blot结果与免疫组织化学结果相一致。结论淫羊藿、黄芪、葛根有效组分复方可以下调AD模型小鼠大脑皮质DMT1的表达,从而抑制小鼠脑铁超载,缓解铁超载带来的中枢神经系统功能衰退。

Objective To investigate the effects of Epimedium,Astragalus,Radix Puerariae on DMT1 expression in the cerebral cortex of APPswe/PS1△E9 double transgenic mice model of AD. Methods A total of 60 specific-pathogen-free male APPswe/ PSl△E9 double transgenic mice aged 6 months were equally and randomly assigned to model, Epimedium, Astragalus, Radix puerariae,compound and DFO groups. An additional 10 6-month-old C57BL/6J mice served as negative control group. Using immunohistochemistry and molecular biology methods to investigate the effects of a compound combining the effective components of Epimedium, Astragalus, Radix puerariae on DMT1 expression in the cerebral cortex of APPswe/PSl△E9 double transgenic mice model of AD. ResUlts Immunohistochemical staining results revealed that DMT1 positive cell did not show in negative control group. DMT1 expression was higher in mode[ group compared with the negative control group. DMT1 expression was lower in the compound and deferoxamine groups than in the model group. No significant difference was detected in DMT1 expression between deferoxamine and compound groups. RT-PCR, Western blot and immunohistochemical staining results showed no significant difference. Conclusion These compounds can downregulate DMT1 expression and inhibit iron overload in the cerebral cortex of mice with Alzheimer＇s disease,reduce iron overload induced impairment of the central nervous system.