具有抗骨质疏松作用的N16蛋白的生物工程制备工艺研究

Preparation of N16,an anti-osteoporosis protein,by biological engineering method

N16蛋白具有抑制破骨和促进成骨作用,对骨质疏松疾病的治疗具有潜在的应用价值。采用pRHis质粒和BL21(DE3)plysS菌株,建立了表达产量较高的N16-pRHis-BL21(DE3)plysS表达体系;并建立了N16蛋白分离纯化工艺。该工艺表达方式简单,分离纯化操作简便,蛋白产量高,成本低,周期短,为进一步研究N16蛋白的功能奠定了基础。

N16 protein is a potential agent for treatment of osteoporosis due to its favorable anabolic and antiresorptive effects on bone. Our work described a biological engineering method to prepare N16. To build the high level expression system,pRHis plasmid was transformed into BL21（ DE3） plys S strain.The present study has established a complete N16 preparation process with simple protein expression and purification procedures. This procedure can improve N16 protein production,reduce the cost and shorten the cycle,which will facilitate further studies on the functions of N16.