野生一粒小麦根干旱响应蛋白的筛选与鉴定

Screening and Identification of the Proteins Related to Drought in Root of T.boeoticum

为解析野生一粒小麦根响应干旱胁迫的调控网络,用20%(w/v)PEG6000处理两叶一心期野生一粒小麦,通过对小麦根蛋白质的分离和鉴定,分析了干旱胁迫下野生一粒小麦根中的蛋白质变化。结果共分离、鉴定得到85个表达差异倍数在1.5以上的蛋白质。这些差异蛋白主要涉及碳代谢(27%)、氨基酸代谢(15%)、解毒与防御(11%)、分子伴侣(8%)、能量代谢(8%)、信号传导(6%)、蛋白质代谢(5%)、脂代谢(4%)、转录与翻译(4%)、核苷酸代谢(2%)以及骨架蛋白(1%)。对这些表达差异蛋白进行进一步的功能分析,发现大部分信号传导相关蛋白上调表达;碳代谢途径中的糖酵解相关蛋白下调表达,而磷酸戊糖途径相关蛋白上调表达。同时下调表达的还包括蛋白质代谢相关蛋白。大部分氨基酸代谢相关蛋白质下调表达,但是谷氨酸脱羧酶及甲硫氨酸合酶含量上升。结果表明,干旱胁迫增强了野生一粒小麦根中信号传导与磷酸戊糖途径代谢,促进了谷氨酸与甲硫氨酸的生物合成,降低了糖酵解与蛋白质代谢等过程。研究结果丰富了野生一粒小麦根响应干旱胁迫机制信息。

To uncover the regulation network of Triticum boeoticum under drought stress,a proteomic approach was applied to identify the proteins relative to drought response in root of Triticum boeoticurn. About two-week old seedlings of Triticurn boeoticurn （at three leaves stage） were treated with 20%（w/v） PEG6000. Proteins in root were isolated through two dimensional electrophoresis and indentified by mass spectrum. Eighty-five differential expressed proteins（DEPs） with at least 1.5-fold change in abundance were detected on the 2-DE gels. These proteins were mainly associated with carbon metabolism （ 2 7 % ）, amino acid metabolism （ 15 % ）, detoxification and defense （ 11% ）, chaperones （8 G ）, energy metabolism （8 % ）, signal transduction （ 6 % ）, protein＂ metabolism （ 5 G ）, lipid metabolism （4 - ）, transcription and translation （4 % ）, nucleotide metabolism （2 % ）, and cyto skeleton and cytokinesis（1%）. Further functional analysis revealed that signal sensing and transduetion-associated proteins were greatly up-regulated in the root. Glycolysis was down-regulated but PPP pathway enhanced in the root, resulting in the occurrence of complex changes in energy metabolism. Protein metabolism was down-regulated in the root,as well as most of amino acid metabolism associated proteins. However, the intensity of glutamate decarboxylase and methionine synthase was increased after drought stress. These results would contribute to the existing knowledge on the complexity of root protein changes that occur in response to drought.