脉冲场凝胶电泳在副溶血性弧菌食物中毒的同源性分析

Homology Analysis of Vibrio Parahaemolyticus Isolates from Food Poisoning Outbreak Using Pulsed-Field Gel Electrophoresis

目的应用脉冲场凝胶电泳(PFGE)技术,对一起食物中毒分离的副溶血性弧菌进行同源性分析。方法将食物中毒患者肛拭标本中分离的7株副溶血性弧菌用限制性内切酶NotⅠ酶切后进行PFGE分子分型,用BioNumerics软件对PFGE分型图谱进行聚类分析。结果患者样本分离的7株副溶血性弧菌PFGE图谱条带一致,相似性为100%。结论脉冲场凝胶电泳技术适用于食物中毒事件分离菌株的同源性分析。

Objective To conduct the homology analysis of Vibrio parahaemolyticus strains isolated from a food poisoning outbreak using pulsed-field gel electrophoresis technology（PFGE）.Methods Molecular subtyping of 7Vibrio parahaemolyticus strains isolated from anal swabs of a patient with food poisoning was performed by PFGE after NotⅠdigestion.The DNA fingerprint maps of different Vibrio parahaemolyticus strains were analyzed by BioNumerics software.Results PFGE patterns of the 7strains showed a 100% similarity.Conclusion PFGE is applicable to the homology analysis of isolates from food poisoning outbreak.