HPLC法测定丹酚及其滴丸中丹酚酸的含量

Determination of Salvianolic Acids in Danfen and Its Dropping Pills by HPLC

目的建立测定丹酚及其滴丸中6种主要丹酚酸含量的方法。方法采用HPLC法,Unitary C18色谱柱,乙腈-0.05%磷酸水为流动相梯度洗脱,检测波长286 nm,流速为1.1 ml·min^-1,柱温为35℃。结果该色谱条件下,6种酚酸类成分可完全分离。丹参素、原儿茶醛、迷迭香酸、紫草酸、丹酚酸B和丹酚酸A的线性范围分别为2.07~20.66μg·mL^-1（r=0.9998）、2.53~25.30μg·mL^-1（r=0.9999）、8.12~40.60μg·mL^-1（r=0.9999）、11.24~56.20μg·mL^-1（r=0.9999）、99.60~996.00μg·mL^-1（r=0.9999）、1.00~10.02μg·mL^-1（r=0.9998）,6种成分的平均回收率为99.24%~101.55%,RSD均小于2%。结论该测定方法准确度高,重复性好,专属性强,可同时测定丹酚及其滴丸中6种丹酚酸的含量。

Objective To establish a method for determining six main phenolic acids in extracts of Salvia miltiorrhiza. Methods Unitary C18 column was used with acetonitrile-0. 05% H3PO4 water at a dectection wave-length of 286 nm. The flow rate was 1. 1 ml·min^- 1and the column temperature was 35 ℃. Results Linearity was obtained over the range of 2. 07- 20. 66 μg·mL^-1（ r =0. 9998） for Danshensu,2. 53-25. 30 μg·mL^-1（ r =0. 9999） for portoeaetehualdehdye,8. 12- 40. 60 μg·mL^-1（ r =0. 9999） for rosmarinic acid,11. 24-56. 20 μg·mL^-1（ r = 0. 9999）for lithospermic acid,99. 60- 996. 00 μg·mL^-1（ r = 0. 9999） for salvianolic acid B,1. 00- 10. 02 μg·mL^-1（ r =0. 9998） for salvianolic acid A. The average recovery of six phenolic acids ranged from 99. 31% to 101. 16%,RSD ﹤2%. Conclusion This method is accurate,reproducible and specific,which can be used to determine six main phenolic acids in water extracts of salvia miltiorrhiza and its dropping pills simultaneously.