中国南方汉族人群血浆谷胱甘肽过氧化物酶基因启动子区-991C/T基因多态性与脑梗死的关系

The correlation between GPX- 3 gene promoter polymorp Hisms of Han population in southern China and cerebral infarction

目的研究南方汉族人群谷胱甘肽过氧化物酶(GPX-3)基因启动子区-991C/T基因多态性的分布及其与脑梗死的关系。方法检测佛山市南海区第二人民医院神经内科自2011年11月至2015年6月收治的南方地区汉族脑梗死患者112例(病例组)和同期南方地区汉族健康体检者112例(对照组)的GPX-3基因启动子区-991C/T基因多态性,比较两组的一般资料、卒中危险因素及-991C/T基因多态性的分布特点,分析影响脑梗死发生的危险因素。结果与对照组比较,病例组高血压病史、血脂、尿酸、血同型半胱氨酸、血糖水平有明显差异(P<0.01),-991C/T TT基因型频率及T等位基因频率均较高,差异有统计学意义(P<0.05)。结论中国南方地区汉族人群GPX-3基因启动子区-991C/T位点存在多态性,TT基因型或为脑梗死的易感基因型。

Objective To investigate the relationship between- 991C/T single nucleotide polymorp Hisms（ SNPs） of GPX- 3 gene promoter and cerebral infarction（ CI）,analyze the Genotypes frequency and allele frequency distribution. Methods By using a case- control method,112 ACI patients and 112 healthy subjects of Chinese Han populations of Southern China,were recruited in the study. Genotypes of GPX- 3promoter SNPs- 991 C / T were identified by the polymerase chain reaction and restriction fragment length polymorp Hism. The genotypes and alleles of the SNPs were analyzed to investigate the distribution characteristics between the normal population and patients with CI,and the risk factors induced death. Results The differences of history of hypertension,concentrations of blood fat,urine acid,Hcy and blood sugar between the two groups were statistically significant（ P 0. 05）. The TT genotype frequency（ 66. 96%） in cases was higher than that in controls（ 47. 32%）,and the difference was significant（ P = 0. 003）. Conclusions The C allele of SNP-991 C / T in promoter region of GPX- 3 in southern Han population proved to have polymorp Hism. TT genotype may be susceptible genotype of cerebral infarction.