噬菌体治疗铜绿假单胞菌感染的初步应用研究

Preliminary application of bacteriophages against Pseudomonas aeruginosa infection

目的为铜绿假单胞菌感染提供一种新的治疗方法。方法本研究分别利用铜绿假单胞菌临床标准株建立小鼠全身感染模型,再应用其噬菌体建立治疗模型。结果噬菌体宿主特异性研究结果显示,该噬菌体具有宿主特异性,可以在铜绿假单胞菌平板上形成噬菌斑,不能在大肠埃希菌和金黄色葡萄球菌平板上形成噬菌斑。噬菌体治疗铜绿假单胞菌导致的小鼠全身感染模型,具有较好的效果,但是要控制剂量。治疗后,小鼠的存活率有所上升,小鼠肝脏水肿、充血症状减轻。结论噬菌体治疗细菌感染性疾病的方法是具有可行性的,有望成为抗生素治疗细菌感染的替代疗法。

Objective To provide a new treatment for Pseudomonas infection. Methods In this study we established a mice model of systemic infection with clinical standard strains of Pseudomonas aeruginosa,and then to build a therapy model with its bacteriophages. Results Specificity study on phage host showed that the phage had a host specificity,which could form plaques on the plates of Pseudomonas aeruginosa,but couldn＇t on Ecoli and S. aureus plates. It had a better effect on the systematic infected mices by Pseudomonas aeruginosa,but the dose should be controlled. After treatment,the survival rate of the mice increased,and liver edema,congestive symptoms improved. Conclusion Bacteriophage treating bacterial infected diseases is feasible,and it＇s expected to become an alternative of antibiotic therapy on bacterial infections.