隆朋对大鼠不同脑区5-HT及5-HIAA的影响

Effects of Xylazine on the 5-HT and 5-HIAA contents in different encephalic of rats

研究隆朋对大鼠不同脑区抑制性单胺类神经递质含量的变化影响,探讨隆朋麻醉作用机制。本试验将32只大鼠随机分为四组,分别为对照组,麻醉组,恢复Ⅰ组和恢复Ⅱ组,使用高效液相色谱法检测不同麻醉时期不同脑组织的5-羟色胺(5-HT)和5-羟吲哚乙酸(5-HIAA)的含量。结果隆朋作用后,大鼠大脑皮层、海马和丘脑5-HT和5-HIAA含量分别升高,且差异极显著。表明这三个脑区是隆朋作用脑组织的靶位区,由此推断隆朋可以通过促进海马、丘脑和大脑皮层内神经突触释放5-HT和5-HIAA而产生麻醉作用。

To study the effect of xylazine changes on different brain regions of the inhibitory neurotransmitter in rats,and explore the mechanism of xylazine. 32 rats were randomly divided into control group,maintenance of anesthesia group,recoveryfrom-anesthesia I group,recovery-from-anesthesia Ⅱ group,and the contents of 5-HT and 5-HIAA in different encephalic regions in rats were measured by high performance liquid chromatography-fluorescence.The contents of 5-HT and 5-HIAA in cerebrum,hippocampus and thalamus significantly increased.The results indicated that the targets of xylazine in brain were cerebrum,hippocampus and thalamus.In conclusion,xylazine plays an anesthetic role by increasing 5-HT and 5-HIAA releasing in cerebral hippocampus and thalamus.