大麦-DDGS型日粮中添加非淀粉多糖酶对肉鸡养分利用和肠道健康的影响

Effect of non-starch polysaccharide enzymes in the barley-DDGS diet on nutrient utilization and gut health of broiler

试验旨在研究大麦-DDGS日粮中添加非淀粉多糖酶对肉鸡养分利用和肠道健康的影响。选用960只一日龄的AA肉鸡随机分成8组,每组6个重复,每个重复20只鸡。第1组为正对照,饲喂常规能量日粮;第5组为负对照,饲喂低能量（低250.8 k J）日粮。2～4组与6～8组为试验组,分别在正对照组与负对照组的基础上添加100、200、300 g/t 3个水平梯度的非淀粉多糖酶。结果表明：①1～21 d,低能试验组T8的蛋白利用率显著高于负对照组T5（P〈0.05）;22～42 d,低能试验组T8的蛋白和能量代谢率均显著高于负对照组T5（P〈0.05）。酶制剂水平在300 g/t时,蛋白、能量代谢率提高最明显。②21 d,常规试验组T4的肌胃相对重量显著低于对照组T1（P〈0.05）;42 d,常规试验组T4的空肠、回肠相对重量显著低于对照组T1（P〈0.05）,低能试验组T8的空肠、回肠相对重量显著低于负对照组T5（P〈0.05）。酶制剂水平在300 g/t时,肠道健康改善效果最佳。研究表明,分别在常规和低能（低250.8 k J）大麦-DDGS日粮中添加复合非淀粉多糖酶能够有效提高肉鸡蛋白和能量利用率,改善肉鸡的肠道健康,并且在酶制剂添加300 g/t水平时效果最佳。

The objective of this experiment was to assess the effect of non-starch polysaccharide（NSP）enzymes in barley-DDGS diet on the nutrient utilization and gut health of broiler. Nine hundred andsixty 1-day-old healthy AA broilers were randomly divided into 8 treatments with 6 replications（20×6×8=brids×replications×treatments）. The group 1 was fed a regular energy diet as positive control group;the group 5 fed an energy-restricted diet that 250.8 k J energy less than the conventional diet as a neg-ative contrast group, the group of 2～4 added 100, 200, 300 g/t of non-starch polysaccharide enzymeson the basis of positive control group, and the group of 6～8 added that on the basis of negative con-trast group. The results showed as follows： ①In 1～21 d, the protein utilization ratio of group 8 wassignificantly higher than group 5（P〈0.05）; In 22～42 d, the protein and energy utilization ratio of group8 were significantly higher than that of groups 5（P〈0.05）. The optimal supplementation of NSP en-zymes in a barley-DDGS diet for broilers was 300 g/t, which the nutrient digestibility raised most obvi-ously. ② On 21 d, the relative weight of glandular stomach of group 4 was lower than that of group 1（P〈0.05）; On 42 d, the relative weight of jeju-num and ileum in group 4 were significantly low-er than that in group 1（P〈0.05） and that ofgroup 8 was significantly lower than that ofgroup 5（P〈0.05）. The optimal supplementation of NSP enzymes in a barley-DDGS diet for broilers was 300 g/t, which made the favorable effect of di-gestive organs. The current study suggested that the NSP enzymes significantly improved the nutrientutilization and digestive organs of broiler respectively in conventional and low energy（low 250.8 k J）barley-DDGS diet, and the optimal supplementation of NSP enzymes was 300 g/t in the condition ofthis experiment.