人工湿地去除畜禽养殖废水中磺胺类抗生素抗性基因研究

Removal of Sulfonamide Resistance Genes in Livestock Farms with Constructed Wetland

采用垂直潜流人工湿地研究了畜禽养殖废水中磺胺类抗生素抗性基因(ARGs)在人工湿地中的去除及累积情况。结果表明,畜禽养殖废水中的3种磺胺类ARGs(sulⅠ,sulⅡ及sul Ⅲ)的平均绝对含量分别为1.15×10^(10)、7.51×10^(10)及7.51×10~7copies/L。通过湿地系统处理后sulⅠ、sul Ⅱ及sulⅢ的平均去除率分别为89%、88%及84%。在系统运行末期,湿地表层土壤和底层土壤中sulⅠ、sulⅡ及sulⅢ的绝对拷贝数和相对表达量均有明显的升高。结果表明,人工湿地系统可有效降低畜禽养殖废水中ARGs含量。

This study investigated the efficiency and accumulation of vertical flow constructed wetland on removing sulfonamide resistance genes（ ARGs） from livestock wastewater. The results showed that the average absolute abundances of sulⅠ,sulⅡ and sul Ⅲ were 1. 15 × 10^10,7. 51 × 10^10 and 7. 51 × 10^7 gene copies per liter,respectively. Vertical flow constructed wetland could significantly reduce the content of wastewater ARGs,and average removal rates were 89%,88% and 84%,respectively.Compare to the content of ARGs in unpolluted soil,the content of that in soil of the wetland had an obvious increase at the end stage of this study. In summary,the wetland could effectively reduce the content of ARGs in livestock farm wastewater.