超高效液相色谱-三重四极杆质谱法检测复方阿胶补血颗粒中的阿胶

Qualitative Detection of Donkey-hide Gelatin in Fufang Ejiao Buxue Granule by UPLC-QQQ / MS

目的:建立复方阿胶补血颗粒中阿胶的专属性检测方法。方法:采用胰蛋白酶对复方阿胶补血颗粒处方中阿胶成分进行酶解,利用超高效液相色谱-三重四极杆质谱对阿胶特征分子离子峰进行检测。结果:10批市售样品中均可检出阿胶的特征分子离子峰。结论:所建立的方法经方法学验证,该方法专属性强,可用于复方阿胶补血颗粒中阿胶的检测,为含阿胶药材的中成药的质量控制及标准研究提供参考。

Objective： To establish an analytical method for the identification of donkey-hide gelatin in Fufang Ejiao Buxue gran- ule. Methods： The identification of donkey-hide gelatin in Fufang Ejiao Buxue granule was established by rapid resolution liquid chro- matography （UPLC） coupled with triple quadruple mass spectrometry （QQQ-MS）. Results： The characteristic molecular peaks of donkey-hide gelatin were detected in ten batches of commercial samples. Conclusion： The present method is specific, precise and reli- able, and suitable for the identification of donkey-hide gelatin in Fufang Ejiao Buxue granule. The method provides scientific reference for the study of quality control method for gelatin ingredients in Chinese patent medicines.