摘要
目的:建立高效液相色谱法测定金沙牛化石片中夏佛塔苷的含量。方法:采用Agilent ZORBAX SB-C_(18)色谱柱(150mm×4.6 mm,5μm),以0.2%磷酸-乙腈为流动相进行梯度洗脱,流速为1.0 ml·min^(-1),检测波长为272nm,柱温为25℃。结果:夏佛塔苷在4.58~183.15μg·ml-1浓度范围内与峰面积呈良好线性关系(r=1.000 0),平均加样回收率为99.65%(n=6),RSD为1.09%。结论:该方法简单、准确,具有良好的重复性和稳定性,可用于金沙牛化石片中夏佛塔苷的含量测定。
Objective： To establish an HPLC method to determine the content of schaftoside in Jinshaniu Huashi tablets. Meth- ods： The separation was performed on an Agilent ZORBAX SB-C18 column （ 150 mmx 4.6 ram, 5 μm）with 0.2% phosphoric acid- aeetonitrile as the mobile phase with gradient elution at a flow rate of 1.0 ml ·min-1. The detection wavelength was set at 272 nm, and the column temperature was set at 25 ℃. Results： The calibration curve of sehaftoside had a good linearity within the range of 4.58-183.15μg · m1-1 （r = 1. 000 0）. The average recovery was 99.65% with RSD of 1.09% （n =6）. Conclusion： The established method is simple and accurate with high repeatability, which can be used for the content determination of schaftoside in Jinshaniu Hua- shi tablets.
出处
《中国药师》
CAS
2016年第6期1224-1225,共2页
China Pharmacist
基金
东莞市科技计划项目
