光照波长和光子照度对霜鹿角珊瑚(Acropora pruinosa)生长及代谢的影响

Effect of Light Intensity and Spectral Quality on Growths and Metabolic Response of Acropora pruinosa

在实验室条件下设置380~410、430~460、530~560、610~640 nm等4种波长,以及60、120、180、240、300、360μmol·m^(-2)·s^(-1)等6组梯度光子照度,分别观察单枝霜鹿角珊瑚在不同光谱波长、光子照度条件下珊瑚的生长特性和代谢水平。结果表明:光子照度240μmol·m^(-2)·s^(-1)的不同光谱条件下,霜鹿角珊瑚平均生长率(G)、单位面积叶绿素含量(Nchl-a)、蛋白质(ω_P)、脂质(ω_L)、碳水化合物(ω_C)质量分数变化呈显著性差异(P<0.05),除ω_C以外,均在波长530~640 nm光谱光照条件下达到最大值,其中ω_L是ω_P和ω_C的10倍以上;不同光谱、光子照度梯度条件下,光照条件下的钙化率(G_L)、净光合作用效率(P_N)、总光合作用效率(P_G)差异具统计学意义(P<0.05),在一定范围内,G_L、P_G、P_N随着光子照度增强而升高,当光子照度超过光合效率光饱和值时,停止升高或开始降低;G_L/G_D和G_L/P_G值也随着光子照度增强而升高,变化范围分别在1.51~7.05、0.26~0.69之间,但P_G/P_N值随着光子照度增强而降低,变化范围在0.69~7.38之间。

Nubbins from Acropora pruinosa were dissected and cultured unifactor and control experiment.By the survey of growth characteristics and metabolic level under a range of light wavelength and light intensities,the results suggest the four light spectral（40 μmol photons m^-2s^-1）have significant impact on the Mean growth rates（G）, areal zooxanthellae density（NZ）and zooxanthellae chlorophyll-a content（Nchl-a）,ratio of Protein（ωP）,Carbohydrate（ωC）and Lipid content（ωL）, indicating significant differences between control and treatment（P〈0.05）. Both parameters of peaked at 530-640 nm,except Carbohydrate. Lipid content to almost 10-fold higher values for the Protein（ωP）and Carbohydrate（ωC）. The light-dependant calcification rates（GL）,net photosynthesis（PN）and gross photosynthesis（PG）show significant difference among them under a range of light wavelength and light intensities（P〈0.05）. the increases of light-dependent calcification rates（GL）,gross photosynthesis（PG）and net photosynthesis（PN）along with the increase of light intensities,and begin to stop increase or decrease when the light intensities reach their peak at a threshold that was found between the light intensity and photosynthetic efficiency. Accordingly,the ratio of light-dependant calcification rates（GL）to dark calcification rates（GD）and light-dependant calcification rates（GL）to gross photosynthesis（PG）remains a similar variation curve and the ratio ranges from 1.51 to 7.05 and 0.26 to 0.69. However,the ratio gross photosynthesis（PG）to net photosynthesis（PN）begin to decrease along with the increase of light intensities and the ratio ranges from 0.69 to 7.38.