响应面法优化超声波—微波协同提取富硒蛹虫草硒多糖工艺

Optimization of Ultrasound-Microwave Assisted Extraction of Selenium-Containing Polysaccharides from Se-Enriched Cordyceps militaris by Response Surface Methodology

在单因素试验基础上,采用响应面法对富硒蛹虫草硒多糖超声波-微波协同提取工艺进行优化,并与超声波提取法和水提法提取硒多糖抗氧化活性进行比较分析,探究3种提取方法对硒多糖提取效果的影响。结果表明:超声波—微波协同提取最佳工艺条件为超声时间26.0 min、微波时间3.20 min、微波功率350 W、液料比32.00∶1(mL/g),在此条件下,硒多糖提取率为5.05%,比超声提取法和水提法分别提高了19.96%和3.70%;3种提取方法硒多糖体外抗氧化活性排序依次为:超声波—微波协同提取法>超声波提取法>水提法。此外,超声波—微波协同提取法可获得更高的多糖硒含量,为360.37 mg/kg,相比超声提取法和水提法分别提高了4.47%和12.92%。

Response surface methodology was used to optimize the ultrasound-microwave assisted extraction（UME） of selenium-containing polysaccharides from Se-enriched Cordyceps militaris（SCMP） based on single factor experiments. The yield, selenium content and antioxidant activities in vitro of SCMP were compared with those obtained with hot water extraction（HWE） and ultrasonic extraction（UE）. The results indicated that the optimum conditions for UME extraction were as follows： ultrasonication time, 26.0 min; subsequent microwave treatment, 3.20 min; microwave power, 350 W; and ratio of liquor to solvent, 32.00：1（mL/g）. Under these conditions, the extraction yield of SCMP was 5.05%, which was increased by 19.96% and 3.70% compared with UE and HWE, respectively. Furthermore, the SCMP prepared by UME exhibited the strongest antioxidant activity and contained the highest level of organic selenium, and its selenium content was 360.37 mg/kg, which was increased by 4.47% and 12.92% as compared with those obtained with UE and HWE.