摘要
采用铁盐催化比色法、硫酸—香草醛法、盐酸—香草醛法和直接紫外分光光度法对13个品种葡萄枝蔓中原花色素含量进行分析,直接紫外分光光度法选取不同原花色素对照品对测定结果进行比较。通过方法学考察和统计分析,比较不同品种葡萄枝蔓中原花色素分析方法的优缺点。结果发现铁盐催化比色法和香草醛法专一性强,但铁盐催化比色法反应时间长,香草醛法不稳定,而直接紫外分光光度法稳定性好、精密度高、操作简便,适合葡萄枝蔓中原花色素含量的快速测定。采用直接紫外分光光度法比较分析,分别以原花色素对照品、原花色素B_1和原花色素B_2为标准,发现所得各种葡萄枝蔓中原花色素的含量计算结果差别接近2倍,说明原花色素对照品的纯度及组成对原花色素含量的分析结果影响明显。
The contents of proanthocyanidins in grape vines from 13 different varieties were analyzed by four different methods, namely ferric ion catalysis-colorimetry, H2SO4-vanillin assay, HCl-vanillin assay and UV spectrophotometry. The quantitative determination by UV spectrophotometry was carried out using 3 different proanthocyanidin reference substances. The methodological evaluation and statistical analysis showed that ferric ion catalysis-colorimetry was timeconsuming and vanillin assays had poor stability, though both methods had a high specificity. On the other hand, the UV spectrophotometric method was more stable, precise and convenient. For all 13 grape varieties tested, there was an approximately 2-fold difference in proanthocyanidin contents in grape vines assayed by UV spectrophotometry using 3 different reference substances. As a result, the purity and composition of reference substances had a significant influence on the results of proanthocyanidins determination.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2016年第12期169-175,共7页
Food Science
