高效液相色谱法测定小麦中脱氧雪腐镰刀菌烯醇含量的不确定度评定

Uncertainty Evaluation for Determining Deoxynivalenol in Wheat by High Performance Liquid Chromatography

对高效液相色谱法测定小麦中脱氧雪腐镰刀菌烯醇含量的测量不确定度进行评定。依据JJF 1135—2005《化学分析测量不确定度评定》及CNAS-GL06—2006《化学分析中不确定度的评估指南》中不确定度评定的基本程序,建立数学模型,分析高效液相色谱法测定小麦中脱氧雪腐镰刀菌烯醇含量过程中引入的各测量不确定度分量,计算得到测量结果的合成标准不确定度和扩展不确定度。评定结果表明:影响检测结果不确定度的因素主要是质量浓度因素,其次是体积因素及样品非均匀性因素,相对标准不确定度分别为0.040 3、0.019 7、0.019 6。

The measurement uncertainty for the determination of deoxynivalenol in wheat by high performance liquidchromatography （HPLC） was evaluated. According to the procedure for uncertainty assessment described in JJF 1135-2005 Evaluation of uncertainty in chemical analysis measurement and CNAS-GL06-2006 guidance on Evaluatingthe uncertainty in chemical analysis, a mathematical model was established, and the components of uncertainty duringthe determination of deoxynivalenol in wheat by HPLC were analyzed, and then the combined uncertainty and theexpanded uncertainty were calculated. The results showed that the major factor affecting the measurement uncertaintywas concentration while the minor ones were volume and sample heterogeneity. The corresponding relative standarduncertainties were 0.040 3, 0.019 7 and 0.019 6, respectively.