大承气汤对过敏性哮喘小鼠肺组织形态及IgE水平的影响

Effects of Dachengqi Decoction on Pulmonary Histology and Total IgE Levels in Mice with Allergic Asthma

目的观察大承气汤对过敏性哮喘小鼠肺组织形态学变化、肺指数、脾指数以及总Ig E水平的影响,探讨其相关作用机制。方法采用卵白蛋白致敏加激发的方式建立小鼠过敏性哮喘模型。将20只雌性C57BL/6小鼠随机分为正常组、模型组、大承气汤组和阳性药组,各给药组给予相应药物灌胃。测定各组小鼠肺指数、脾指数,ELISA测定血清Ig E含量,HE染色进行病理观察。结果与正常组比较,模型组小鼠肺指数增加,血清Ig E含量明显升高(P<0.01),肺组织出现炎性病理改变;与模型组比较,大承气汤组和阳性药组小鼠肺指数明显降低(P<0.05),血清Ig E含量明显降低(P<0.01),肺组织形态结构明显改善。结论大承气汤对哮喘小鼠肺部炎症有抑制作用。

Objective To explore the effects ofDachengqiDecoction on morphological changes, lung and spleen indexes, and total IgE levels of mice with allergic asthma; To discuss it relevant mechanism of action.MethodsThe method of OVA sensitization and provocation was used to establish allergic asthma mice models. 20 C57BL/6 mice were randomly divided into control group, model group,Dachengqi Decoction group and dexamethasone positive group. Each medication group was given relevant medicine for gavage. Lung and spleen indexes in each group were detected. The total IgE levels in serum of mice were detected by ELISA, and HE staining was used to conduct pathological observation.Results Compared with control group, the lung index and level of IgE in model group increased significantly and pulmonary histological results showed abundant inflammatory cells infiltrated in the bronchus; compared with the model group, the lung index as well as the levels of IgE decreased significantly in the Dachengqi Decoction and dexamethasone positive group (P<0.05), and the lung tissue organization form was improved significantly.ConclusionDachengqi Decoction has inhibiting effects on pulmonary inflammation of mice with asthma.