HPLC波长切换法测定银花芒果片中芒果苷、橙皮苷含量

HPLC Wavelength Switching Simultaneous Determination for the Contents of Mangiferin and Hesperidin in Yinhua Mangguo Tablets

目的 建立HPLC 波长切换法测定银花芒果片中芒果苷、橙皮苷含量的方法.方法 采用AgilentZORBAX Eclipse XDB-C18（4.6 mm×150 mm,5 μm）色谱柱,流动相为甲醇-0.2%磷酸水溶液,梯度洗脱,流速为1.0 mL/min,柱温30 ℃,波长切换时间序列采样（0～20 min 为258 nm,20～50 min 为283 nm）.结果 芒果苷进样量在0.042～0.761 μg 范围内线性关系良好（r=0.999 9）,平均回收率为99.86%,RSD=1.5%（n=9）;橙皮苷进样量在0.076～1.361 μg 范围内线性关系良好（r=0.999 8）,平均回收率为99.91%,RSD=0.7%（n=9）.结论 本方法简便、快捷、准确,可用于该制剂的质量控制.

Objective To establish an HPLC method of wavelength switching simultaneous determination for the contents of mangiferin and hesperidin inYinhua Mangguo Tablets.Methods Agilent ZORBAX Eclipse XDB-C18 (4.6 mm×150 mm, 5 μm) column was used. The mobile phase was methanol-water containing 0.2% phosphoric acid with gradient elution mode at the flow rate of 1.0 mL/min; the detection wavelength was 258 nm (0–20 min) for mangiferin, 283 nm (20–50 min) for hesperidin.Results Mangiferin had a good linearity in the range of 0.042–0.761 μg, and the average recovery was 99.86%, with RSD of 1.5% (n=9). Hesperidin had a good linearity in the range of 0.076–1.361 μg, and the average recovery was 99.91%, with RSD of 0.7% (n=9).Conclusion The method is simple, accurate and reproducible, which can be used to the quality control ofYinhua Mangguo Tablets.