喜马拉雅旱獭源鹑鸡肠球菌的分离及其耐药性检测

Isolation and antibiotic resistance detection of Enterococcus gallinarums from Marmota himalayana

目的了解青藏高原喜马拉雅旱獭携带院内感染病原菌鹑鸡肠球菌情况,并检测其对临床常用抗菌药物的耐药性。方法选择性培养基、革兰染色、生化反应和16S rRNA基因序列分析方法对细菌进行分离和鉴定;K-B纸片法检测菌株对15种抗菌药物的耐药情况,并应用PCR方法检测毒力基因和耐药基因。结果 79份喜马拉雅旱獭肠道样本中共分离到3株鹑鸡肠球菌,其中1株对利福平中介,3株均对奎奴普丁中介,对其他被检抗菌药物均敏感;未检测到常见肠球菌毒力基因(asa1、esp、hyl、gel E和cyl A)和相关耐药基因。结论首次在青藏高原旱獭粪便中分离到鹑鸡肠球菌,常见的毒力基因检测阴性,对常见抗菌药物敏感。

Objective To understand whether Marmota himalayana is a possible carrier of Enterococcus gallinarum,one of the emerging pathogens for hospital related infections. Methods Selective medium culture,biochemical reaction and 16 S rRNA sequencing were conducted for the isolation and identification of isolated strains,K-B test was done to detect the drug susceptibility of isolated strains,and PCR amplification of virulence genes were performed. Methods Three strains of E. gallinarum were isolated from intestinal samples of 79 Marmota himalayana captured in Qinghai-Tibet Plateau in Qinghai province,which were identified by both biochemical tests and 16 S rRNA gene sequence analysis. The drug susceptibility test using 15 common antibiotics indicated that 3 isolates were all sensitive to 13 antibiotics,but intermediate to quinupristin,and1 isolate was intermediate to rifampin. And none of 5 recognized virulence genes in genus of Enterococcus was detected in the3 isolates,i. e. asa1,esp,hyl,gel E and cyl A. Conclusion In contrast to other studies,we isolated 3 antibiotics sensitive and virulence-gene-free E. gallinarum strains from Marmota himalayana for the first time.