摘要
目的建立高效液相色谱法同时测定注射用复合辅酶中辅酶A和辅酶Ⅰ含量。方法色谱柱为Intersil ODS-3(150 mm×4.6 mm,5μm),流动相为甲醇-pH 6.5磷酸二氢钾溶液(梯度洗脱),检测波长为259 nm,流速为1.0 mL·min^(-1),柱温为30℃。结果辅酶A和辅酶Ⅰ的检测浓度分别在1.62~32.48 u·mL^(-1)(r=1.000)、2.14~34.30μg·mL^(-1)(r=1.000)与各自的峰面积呈良好的线性关系;平均加样回收率分别为101.8%(n=6)、101.8%(n=6),RSD分别为2.6%、2.2%。结论本方法简便、准确、重现性好,可用于注射用复合辅酶的质量控制。
Objective To develop an HPLC method to determine coenzyme A and coenzyme Ⅰ in coenzyme complex for injection. Methods The column was Intersil ODS-3 column（150 mm×4.6 mm, 5 μm） with the mobile phase of methanol and pH 6.5 phosphate buffer solution by gradient elution. The detection wavelength was 259 nm, the flow rate was 1.0 mL·min^（-1）, and the column temperature was 30 ℃. Results There was a good linearity between the concentration of coenzyme A and coenzyme Ⅰ and peak area value when the linearity of coenzyme A and coenzyme Ⅰ was 1.62- 32.48 u·mL（-1）（r = 1.000） and 2.14- 34.30 μg·mL（-1）（r =1.000）, respectively. The recoveries of coenzyme A and coenzyme Ⅰ were 101.8%（n = 6） and 101.8%（n = 6）. The RSD values of precision were 2.6% and 2.2%. Conclusion The method is simple, accurate and reproducible, and it can be used for the quality control of coenzyme complex for injection.
出处
《中南药学》
CAS
2016年第4期421-424,共4页
Central South Pharmacy
