阿霉素性慢性心衰大鼠模型不同方案的比较

Comparison of different rat chronic heart failure models induced by adriamycin

比较利用不同方案尾静脉注射阿霉素建立Wistar大鼠慢性心力衰竭模型,评价出较优方案.成年雄性Wistar大鼠50只,随机分为3组:正常对照组(n=10)、模型A组(n=20)、模型B组(n=20).模型A组按2 mg/kg体重剂量尾静脉注射阿霉素,每周1次,共6周;模型B组按3 mg/kg体重和1 mg/kg体重交替给予尾静脉注射盐酸阿霉素,每周1次,共6周;对照组注射相同体积的氯化钠注射液.末次注射停药后2周,测量体重(BW)、心室质量(VW)、左室舒张末期内径(LVEDD)、左室收缩末期内径(LVESD)、左室射血分数(LVEF)、左室短轴缩短率(FS)的变化.结果表明,与对照组相比,模型A组和模型B组VW/BW、LVESD、LVEF显著大于对照组;模型A组和模型B组比较,模型A组LVEF与对照组及模型B组比较有统计学意义.尾静脉交替注射一定剂量的阿霉素是建立大鼠慢性心力衰竭模型的一种简便可靠的方法.

The optimal scheme was evaluated by comparing the different schemes of intravenous injection of doxorubicin to establish the model of chronic heart failure in Wistar rats. A total of 50 male Wistar rats were randomly divided into 3 groups： normal control group（ n =10）,Model A group（ n = 20）,model B group（ n = 20）. Model A group rats were injected doxorubicin one times a week and 2 mg/kg weight dose in 6 weeks. Model B group rats were given by 3 mg/kg weight and 1 mg/kg weight alternately. The control group rats were injected saline. Two weeks after the last injection,measured the body weight（ BW）,left ventricular mass（ VW）,left ventricular end diastolic diameter（ LVEDD）,left ventricular end systolic diameter（ LVESD）,left ventricular ejection fraction（ LVEF）,left ventricular fractional shortening（ FS）. Thr results showed that the VW/BW,LVESD,and LVEF of control group were significantly higher than group model A and group model B. The LVEF of A group was statistically significant lower than Model B group and control group. The method of intravenous alternating injection of a certain dose of doxorubicin was a simple and reliable method to establish a rat model of chronic heart failure.