RACK1高表达促进人结肠癌细胞的生长和增殖

RACK1 overexpression promotes the growth and proliferation of colon cancer cells

目的:研究RACK1高表达对人结肠癌细胞增殖能力的影响。方法:采用脂质体转染术分别建立R ACK1表达下调的S W620细胞系、R ACK1表达上调的S W480细胞系以及对照细胞系;采用CCK-8细胞增殖测定、软琼脂集落形成实验、Ed U掺入实验以及流式细胞术检测RACK1表达改变对S W620和S W480细胞增殖的影响;采用Wester n blot分析R ACK1表达改变对G1/S期限制点调控蛋白Cyclin D1和p27蛋白表达的影响。结果:下调RACK1的表达抑制SW620细胞的生长、软琼脂集落形成能力,降低Ed U标记的S期细胞数目,阻滞细胞周期于G1/S期;而上调RACK1的表达增强S W 4 8 0细胞的生长、软琼脂集落形成能力,增加Ed U标记的S期细胞数目,促进细胞周期G 1/S期进程。结论:RACK1高表达促进人结肠癌细胞的生长和增殖。

Objective： To investigate the effects of RACK1 overexpression on the growth and proliferation of colon cancer cells. Methods： Stable transfected colon cancer cell lines with RACK1 expression changes as well as corresponding control cell lines were established by using Lipofectamine 2000. Cell proliferative ability was analyzed by CCK-8 assay, soft agar colony formation assay, Ed U incorporation assay and flow cytometry. The expression of G1/S phase checkpoint regulation proteins Cyclin D1 and p27 was detected by Western blot. Results： Downregulation of RACK1 in colon cancer SW620 cells inhibited cell growth and soft agar colony formation ability, decreased number of S phase cells labeled by Ed U, and blocked cell cycle at G1/S phase. Overexpression of RACK1 in colon cancer SW480 cells enhanced cell growth and soft agar colony formation ability, increased number of S phase cells labeled by Ed U, and promoted progression of cell cycle G1/S phase. Conclusion： RACK1 overexpression promotes the growth and proliferation of colon cancer cells.