摘要
目的探讨和评价利福平耐药实时荧光定量核酸扩增检测技术（Xpert mycobacterium tuberculosis/rifampicin,Xpert MTB/RIF;简称Xpert检测）与γ-干扰素释放试验在关节结核辅助诊断中的价值。方法选取2015年1~9月首都医科大学附属北京胸科医院住院经手术治疗,病理确诊为关节结核的患者50例,同时行脓液Xpert检测和血酶联免疫斑点技术法（T-SPOT.TB检测）检测结核分枝杆菌感染T细胞。结果 T-SPOT.TB检测在诊断关节结核中的敏感度为88%（44/50）;Xpert检测的敏感度为66%（33/50）;两种方法联合检测的敏感度为66%（33/50）。T-SPOT.TB检测的敏感度高于Xpert检测及两种方法联合检测（χ~2=6.832,P=0.009）。结论 Xpert检测和γ-干扰素释放试验采用T-SPOT.TB检测试剂盒在关节结核快速诊断方面均具有重要的价值,两者结合可提高关节结核诊断的准确度。
Objective To explore and evaluate the diagnositic value of Xpert mycobacterium tuberculosis/rifampicin（Xpert MTB/RIF） and γ-interferon release assays in joint tuberculosis. Method 50 patients diagnosed as joint tuberculosis from January 2015 to September 2015 in Capital Medical University, Beijing Chest Hospital were included into this study. Rifampin puss simultaneously real-time quantitative nucleic acid amplification Xpert MTB/RIF and T-SPOT.TB test were tested at the same time. Result T-SPOT.TB detection sensitivity in the diagnosis of joint tuberculosis was 88%（44/50）; Xpert detection sensitivity was 66%（33/50）; combined detection sensitivity of the two methods was 66%（33/50）. T-SPOT.TB sensitivity is higher than Xpert detection and combined detection of the two methods（χ~2=6.832, P = 0.009）. Conclusion The Xpert MTB/RIF and γ-interferon release assay which applied by T-SPOT.TB detection method have significant value in the rapid diagnosis of tuberculosis joints. A combination of these can improve the accuracy of joint tuberculosis diagnosis.
出处
《中国医刊》
CAS
2016年第6期70-72,共3页
Chinese Journal of Medicine
基金
北京市医院管理局临床医学发展专项(ZYLX201304)
