乌贼墨黑色素对铅中毒小鼠的排铅作用及其对体内钙、锌和铜含量的影响

SEPIA MELANIN: EFFECT ON LEAD REMOVAL AND ON CONTENTS OF CALCIUM, ZINC, COPPER IN MICE

本文研究了乌贼墨黑色素对铅中毒小鼠的排铅作用及对小鼠体内钙、锌和铜含量的影响。采用48只小鼠为实验对象,按体质量随机分为阴性对照组,醋酸铅模型对照组,乌贼墨黑色素低(50mg/kg)、中(100mg/kg)、高(200mg/kg)剂量组以及阳性对照组(1.5g/60 kg),除阴性对照组外其余各组均每天腹腔注射醋酸铅溶液(7mg/kg)10天。第11天起各剂量组用乌贼墨黑色素灌胃,阴性对照组及醋酸铅模型对照组灌胃去离子水,阳性对照组灌胃二巯基丁二酸(DMSA),连续进行28d。采用原子吸收分光光度法测定小鼠的血液、肝、肾及脑组织中的铅、钙、锌和铜的含量。研究发现乌贼墨黑色素高剂量组(200mg/kg)显著降低了血铅含量,下降28.15%;乌贼墨黑色素中剂量组(100mg/kg)显著降低了肝铅含量,下降15.89%。同时,乌贼墨黑色素高剂量组对铅中毒小鼠肝铜、肾锌以及脑锌的含量高于醋酸铅模型对照组(P<0.05),而与阴性对照组相比无统计学差异(P>0.05)。以上结果表明高剂量、中剂量乌贼墨黑色素对血铅与肝铅有一定的排铅作用,同时高剂量的乌贼墨黑色素对小鼠肝脏中的铜、肾脏中的锌以及脑中的锌有保护作用。

We studied the effect of sepia melanin on lead removal and on contents of calcium, zinc and copper in artificially lead-poisoned mice. Forty-eight mice were randomly divided into six groups in body weight： negative control group, lead-poisoned model group, positive control group, and low-, medium-, and high-dosage sepia melanin treated groups. An experiment model of lead-poisoning mice was established by daily intraperitoneal injection with lead acetate solution（7mg/kg） except for the negative control group. They were treated for 10 days. The three treatment groups were administrated intragastrically with 50, 100, and 200mg/kg of sepia melanin. The mice in the positive control group was given dimercaptosuccinic acid（DMSA） solution, and those in negative control group and model control group were given with deionized water by gavage. After the model was run for 11 days, corresponding dosage of sepia melanin was gavaged daily to different groups for 28 days. Afterward, the contents of lead, calcium, zinc, and copper in blood, liver, kidney, and brain of mice were determined in atomic spectrometry. The results show that the lead content in blood of high-dosage melanin treated group was eliminated for 28.15%, and for 15.89% in the liver of the medium-dosage melanin treated group. In addition, copper in liver, and zinc in kidney and brain of the high-dosage group were higher than that in lead-poisoned model group（P〈0.05）. Statistically, there was no significant difference between the negative control and high-dosage group in the contents of copper in liver, zinc in kidney, and brain（P〉0.05）. Therefore, the high- or medium-dosage sepia melanin treatment could effectively remove lead in blood and liver of the mice. Moreover, in the high-dosage group, copper in liver, and zinc in kidney and brain were maintained.