脂联素对重症急性胰腺炎的保护作用及其机制

The protective role of adiponectin on severe acute pancreatitis and its mechanisms

目的:探讨脂联素在重症急性胰腺炎发生发展中的重要作用及其相关机制。方法:60只C57小鼠随机分为脂联素预处理组、脂联素治疗组和对照组,每组20只。以雨蛙肽连续腹腔注射建立重症急性胰腺炎模型,采用病理评分及检测血清淀粉酶、脂肪酶评估胰腺炎症程度,检测血清TNF-α、IL-6、IL-1β水平以评估全身炎症状态,确定脂联素对重症急性胰腺炎的重要作用。对胰腺腺泡细胞AR42J使用脂联素预处理后建立胰腺炎模型,检测通路蛋白NF-κB和促炎因子TNF-α的表达水平,研究脂联素的可能作用通路。结果:建模后,脂联素预处理组和脂联素治疗组小鼠的病理评分显著低于对照组,且血清淀粉酶、脂肪酶及血清TNF-α、IL-6、IL-1β水平也均显著低于对照组(P<0.05)。脂联素预处理后AR42J细胞表达NF-κB及TNF-α水平显著降低(P<0.05)。结论:脂联素可抑制重症急性胰腺炎炎症发展,其可通过下调NF-κB通路及抑制促炎因子TNF-α发挥抗炎作用。

Objective：To investigate the role of adiponectin in the development of severe acute pancreatitis and its mechanisms.Method：Sixty C57 mice were randomly divided into 3groups：adiponectin pretreated group,adiponectin treated group and control group.Severe acute pancreatitis model was built by intraperitoneal injection of cerulein.The pathological score,serum amylase and lipase levels were examined to assess the severity of pancreatic inflammation.And the serum level of TNF-α,IL-6,IL-1βwere examined to assess the severity of systemic inflammation.So the important role of adiponectin in severe acute pancreatitis could be confirmed.Then we used pancreatic acinar cells AR42 Jto build the pancreatitis model after usage of adiponectin.The expression of NF-κB and TNF-αwere examined to investigate the pathways of adiponectin.Result：The pathology scores of mice with severe pancreatitis in the adiponectin pretreated group and adiponectin treated group were significantly lower than that in control group.And the serum amylase,lipase levels,and serum level of TNF-α,IL-6,IL-1βwere also significantly lower in adiponectin pretreated group and adiponectin treated group.The expression of NF-κB and TNF-αin adiponectin group of AR42 Jcells decreased significantly.Conclusion：Adiponectin could inhibit the development of severe acute pancreatitis.And it could downregulate the expression of NF-κB and TNF-αto play the role of anti-inflammatory.