20(S)-原人参二醇-羟丙基-β-环糊精包合物制备工艺优化研究

The preparation process optimization on inclusion complex of 20(S)-protopanoxadiol-hydroxypropyl-β-cyclodextrin

用磁力搅拌法制备20（S）-原人参二醇-羟丙基-β-环糊精包合物,采用高效液相色谱法、正交试验法及红外光谱（IR）、X衍射射线、差示量热分析（DSC）法测定包合物的包封率,以其为指标,筛选最佳包合条件,并验证包合物的形成。结果表明：20（S）-原人参二醇质量浓度为32.75-524.00μg·mL-1时线性关系良好,相关系数r为1,包合的最佳条件为摩尔比1∶1,搅拌时间2h,温度40℃。该方法操作简单、结果准确,可用于羟丙基-β-环糊精包合20（S）-原人参二醇的制备及包封率的测定。

Prepared with a magnetic stirring method 20（S）-protopanoxadiol hydroxypropyl-β-cyclodextrin inclusion complex,high phase liquid chromatography（HPLC）,the orthogonal experiment method,infrared spectrum（IR）,X-ray diffraction rays and differential thermal analysis（DSC）method were used to determine the envelopment rate of inclusion,as indicator,screen and optimize conditions for inclusion,and to verify the formation of inclusion complex.The results showed that 20（S）-protopanoxadiol concentration in 32.75-524μg·mL-1 insider was good,the correlation coefficient of r=1,the best conditions for inclusion were：molar ratio of 1∶1,stirring time 2h,the temperature 40 ℃.The test method was simple,accurate and can be used for hydroxypropyl-β-cyclodextrin inclusion 20（S）-protopanaxatriol preparation and measurement of encapsulation efficiency.