胃癌组织中S100钙结合蛋白基质金属蛋白酶9和基质金属蛋白酶抑制剂的表达及其与临床病理特征的关系

Expression and correlation with clinical pathological features of S100 calcium-binding protein A11,matrix metalloproteinase-9 and reversion-inducing cysteine-rich protein with Kazal motifs in patients with gastric carcinoma

目的探讨胃癌组织中人S100钙结合蛋白(S100A11)、基质金属蛋白酶9(MMP-9)和基质金属蛋白酶抑制剂(RECK)的表达及其与临床病理特征的关系。方法选取2013年7月至2014年7月间收治的62例胃癌患者手术切除的胃癌组织蜡样标本作为胃癌组;采用随机数字表法从广州医科大学附属广州市第一人民医院胃镜检查室内活检提示为正常胃组织中随机选取21例为对照组。采用免疫组化染色方法检测两组标本中S100A11、MMP-9和RECK的表达,比较三者在胃癌组织和正常组织中的表达差异,及其与患者临床病理特征之间的关系。结果胃癌组织中S100A11、MMP-9、RECK的表达阳性率分别为66.1%、75.8%和21.0%,正常胃组织分别为28.6%、19.1%和66.7%,胃癌组织中S100A11、MMP-9表达阳性率均高于正常胃组织(均P<0.05),RECK表达阳性率低于正常胃组织(P<0.05)。随着肿瘤直径的增大、分化程度的增加、淋巴转移的产生、浸润深度的增加,S100A11和MMP-9表达水平显著上升,而RECK表达水平随肿瘤直径的增大、分化程度的增加而上升,随淋巴转移的产生、浸润深度的增加而下降(均P<0.05)。胃癌组织中MMP-9与S100A11的表达呈正相关(r=0.421,P<0.05),而MMP-9与RECK的表达呈负相关(r=-0.284,P<0.05)。结论胃癌组织中S100A11和MMP-9呈高表达,RECK呈低表达,三者与临床病理特征密切相关,检测三者的表达对胃癌的诊治有一定的指导意义。

Objective To explore the expression and correlation with clinical pathological features of S100 calcium-binding protein A11（ S100A11）,matrix metalloproteinase-9（ MMP-9） and reversion-inducing cysteine-rich protein with Kazal motifs（ RECK） in patients with gastric carcinoma. Methods From July 2013 to July 2014 in The First People＇s Hospital of Guangzhou,62 samples of gastric carcinoma and21 samples of normal gastric were chosen and immunohistochemical method was conducted to detect the expressions of S100A11,MMP-9 and RECK. The expressions of S100A11,MMP-9 and RECK in the gastric carcinoma and normal gastric samples were compared and the relationships with clinical pathological features were analyzed. Results The positive expression rate of S100A11,MMP-9 and RECK in gastric carcinoma samples were 66. 1%,75. 8% and 21. 0%,while in normal gastric samples were 28. 6%,19. 1% and66. 7% respectively. The positive expression rate of S100A11 and MMP-9 in gastric carcinoma were both higher than those of the normal gastric sample（ P 〈0. 05）,while it was lower as to RECK（ P 〈0. 05）. As the increasing of tumor diameter,degree of differentiation,infiltration depth and happening of lymph node metastases,the expression level of S100A11 and MMP-9 increased. As the increasing of tumor diameter,degree of differentiation,the expression level of RECK increased. As the increasing of infiltration depth and happening of lymph node metastases,RECK declined. There was a positive correlation between MMP-9 and S100A11（ r = 0. 421,P 〈0. 05）,while a negative correlation between MMP-9 and RECK（ r =-0. 284,P 〈0. 05）. Conclusions The expression of S100A11 and MMP-9 were up-regulated in gastric carcinoma,while RECK was down-regulated. The three markers have a correlation with clinical pathological features which might have a guidance to the diagnose and treatment of gastric carcinoma.