松辽黑猪背最长肌持水力相关基因的分析

Screening differentially expressed genes related to water holding capacity of Songliao black swine

为探讨松辽黑猪背最长肌持水力的分子遗传学机理,利用基因芯片技术对松辽黑猪群体内背最长肌持水力高和低的个体进行了转录分析,利用实时定量PCR对部分转录差异基因进行了检测。差异表达分析显示,共有657个基因显著变化;差异基因经G分类显示,主要参与脂肪细胞因子信号通路、胰岛素信号传导、氨基酸代谢、脂类代谢等生物学过程;所验证的PGM1、PPARGC1α、CXCL14基因与芯片结果基本一致。

In order to investigate the molecular mechanisms underlying porcine water holding capacity（WHC）,the transcriptional profile of longissimus dorsi muscle between lower WHC individuals and higher individuals was compared by microarrays in Songliao black swine,and the differentially expressed genes were verified by real-time PCR.The results showed that 657 differentially expressed genes were found between the two groups.Differentially expressed genes classified by GO（Gene Ontology）analysis mainly involved in adipocytokine signaling pathway,insulin signaling pathway,metabolism of amino acids,lipid metabolism etc.biological progresses.In addition,the real-time PCR results of PGM1,PPARGC1α,CXCL14 were consistent with those of gene chip.These results would offer the basic data for the further study on the molecule genetic mechanism of WHC in Songliao black swine.