血府逐瘀胶囊对内皮损伤大鼠血栓弹力图的影响

Influence of Xuefuzhuyu Capsule on Thrombelastography in Rats with Vascular Endothelial Injury

目的研究血府逐瘀胶囊对高同型半胱氨酸诱导的内皮损伤大鼠血栓弹力图(TEG)的影响。方法 SD大鼠随机分为6组:空白对照组、模型对照组、通心络胶囊0.21 g/kg组、血府逐瘀胶囊1.6、0.8和0.4 g/kg组。2 g/kg蛋氨酸灌胃造模,1次/天,连续4周。造模同时,各治疗组分别灌胃给予相应药物。给药结束后测定大鼠血清同型半胱氨酸(Hcy)、内皮素1(ET-1)和血浆一氧化氮(NO)水平、血小板数目及平均血小板体积(MPV),枸橼酸钠抗凝测TEG。结果血府逐瘀胶囊各剂量组均能降低大鼠血清Hcy和ET-1水平(P<0.01),0.8 g/kg组能够显著升高血浆NO和MPV,降低血小板数目(P<0.05)。血府逐瘀胶囊1.6 g/kg组R值明显升高(P<0.05),MA值显著降低(P<0.01);血府逐瘀胶囊0.8 g/kg组R值明显升高,Angle和MA值显著降低(P<0.01);血府逐瘀胶囊0.4g/kg组MA值明显降低(P<0.01)。结论血府逐瘀胶囊能够明显缓解内皮损伤大鼠血小板功能亢进及高凝血因子状态,对高同型半胱氨酸诱导的血管内皮损伤具有保护作用。

Aim To study the effect of Xuefuzhuyu capsule on thrombelastography（TEG）in rats with vascular endothelial injury induced by homocysteine（Hcy）. Methods SD rats were randomly divided into 6 groups：normal group,model group,Tongxinluo capsule 0. 21 g / kg group,Xuefuzhuyu capsule 1. 6,0. 8 and 0. 4 g / kg groups. Rat endothelial injury models were established by intragastric gavaged of methionine once per day for continuous 4 weeks. Meanwhile,each treated group was intragastric gavaged of corresponding drug. After 4 weeks,serum levels of Hcy and endothelin-1（ET-1）,plasma level of nitric oxide（NO）,platelet（PLT）number and mean platelet volume（MPV）,as well as TEG were detected. Results All doses of Xuefuzhuyu capsule could significantly reduce the serum levels of Hcy and ET-1（P〈0. 01）,increase the plasma level of NO and MPV,and lower the number of PLT number,with statistical significances in 0. 8 g / kg group（P〈0. 05）. All doses of Xuefuzhuyu capsule could significantly reduce MA（P〈0. 01）,the R of 1. 6 and 0. 8 g / kg groups were significantly prolonged（P〈0. 01 or P〈0. 05）,the Angle of 0. 8 g / kg group showed significant decrease（P〈0. 01）. Conclusion Xuefuzhuyu capsule can relieve the state of platelet hyper function and high coagulation factor,and attenuate high Hcy induced injury in rats.