姜百胃炎片对幽门螺杆菌感染模型小鼠的干预作用

Effect of Jiangbai Weiyan Tablets on Helicobacter Pylori Infected Mouse Model

目的观察姜百胃炎片对幽门螺杆菌(Hp)感染模型小鼠体内抑菌作用。方法选用Hp SS1菌株,对实验小鼠(C57BL/6小鼠和BALB/c小鼠)以Na HCO3和混合抗生素预处理、灌胃菌液的方法建立Hp感染小鼠模型。Hp感染成模小鼠随机分成模型组、三联治疗组[雷尼替丁19.5mg/(kg·d),阿莫西林130mg/(kg·d),克拉霉素65mg/(kg·d)]及姜百胃炎片高剂量组[(3.12g/(kg·d)]、中剂量组[(1.56g/(kg·d)]和低剂量组[(0.78g/(kg·d)]。用细菌培养、p H指示剂法、尿素酶试验、抗体试验等评估实验小鼠Hp感染程度。统计方法采用秩和检验。结果 C57BL/6小鼠空白组、模型组、三联治疗组及姜百胃炎片高、中、低剂量组Hp感染程度尿素酶试验平均秩和(R軍i,低优指标)分别为18.50、51.33、18.50、27.32、35.68和38.14,姜百胃炎片高剂量组Hp感染程度显著低于模型组(P<0.05);BALB/c小鼠空白组、模型组、三联治疗组及姜百胃炎片高、中、低剂量组的组的R軍i分别为24.00、58.73、31.17、27.58、32.08和36.58,姜百胃炎片三个剂量组Hp感染程度均显著低于模型组(P<0.05)。结论姜百胃炎片对Hp感染C57BL/6和BALB/c小鼠模型有抑菌作用。

Objective To investigate the anti-helicobacter pylori （Hp） activity of Jiangbai Weiyan tablets on Hp-infected mouse model. Methods The experimental mice （C57BL/6 and BALB/c） were pretreated with NaHCO3 and antibiotics, and inoculated with Hp SS1 strain to establish Hp-infected mouse model. The Hp infected mice were randomly divided into model group, triple therapy group（ranitidine 19.5mg·kg-1·d-1, amoxicillin 130mg·kg-1·d-1, clarithromycin 65mg·kg-1·d-1）, high-dose（3.12g·kg-1·d-1）, mid-dose（1.56g·kg-1·d-1）, and low-dose（0.78mg·kg-1·d-1） groups of Jiangbai Weiyan tablets. Bacterial culture, pH indicator diagnostic test, urease test, and antibody test were used to evaluate the infection extent of experimental mice. Rank sum test was utilized for statistical analysis of the experimental data. Result s R-i values of blank group, model group, triple therapy group, high-dose, mid-dose, and low-dose groups of Jiangbaiweiyan tablets for C57BL/6 mice were 18.50, 51.33, 18.50, 27.32, 35.68 and 38.14, respectively. The infection extent in C57BL/6 mice of high-dose group of Jiangbai Weiyan tablets was lower than that of model group （P〈0.05）. R-i values of blank group, model group, triple therapy group, high-dose, mid-dose, and low-dose groups of Jiangbai Weiyan tablets for BALB/c mice were 24.00, 58.73, 31.17, 27.58, 32.08 and 36.58, respectively. The infection extent in BALB/c mice of all three dose groups of Jiangbai Weiyan tablets was lower than that of model group（P〈0.05）. Conclusion Jiangbai Weiyan tablets can inhibit Hp infection both in C57BL/6 mice and BALB/c mice.